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Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.
Activity of Ziziphora tenuior L. leaves organic extracts against four pathogenic bacteria, which were isolated from clinical samples and prepared from Al-Assad hospital laboratory in Lattakia, were tested by disc diffusion method through 2014. Res ults showed that Ziziphora tenuior L. leaves extracts have antibacterial activity against all Gram positive and negative bacteria. Inhibition zones were recorded between 9mm against Pseudomonas aeruginosa and 44mm against Staphylococcus aureus at concentration of 200mg/ml for the methanolic extract. Whereas the inhibition zones for the ethanolic extract were recorded between 7mm at concentration of 40mg/ml against Streptococcus sp. and 34mm at concentration of 200mg/ml against Staphylococcus aureus. These results represent the importance of Ziziphora tenuior L. as a source of natural substances counteraction of pathogenic bacteria.
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method. Results showed that water extracts had antibacte rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris. All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm. Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm. This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
Lichen (Lecanora epibryon) collected from Arshany reservation at Idleb city was taken for screening antibacterial activity. Acetone was used as solvent for extraction of active compounds from lichen. The activity of extract was tested against thre e isolated infectious bacteria (Pseudomonas sp., Salmonella sp., Klebsiella sp.) by cytotoxicity assay using MTT test. Acetone extract of lichen showed antibacterial activity against all test organisms, but Salmonella sp. was highly susceptible to the extract even at low concentration.
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