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Register a new userActivity of acetone extract from Lichen Lecanora epibryon against some isolated infectious bacteria
فاعلية المستخلص الأسيتوني لأشنة Lecanora epibryon تجاه بعض العزلات الجرثومية الممرضة
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جمعت عينات للأشنات من النوع Lecanora epibryon من محمية العرشاني في مدينة إدلب، بهدف دراسة فاعليتها المضادة للجراثيم، و استعمل الأسيتون كمذيب لاستخلاص المركبات الفعالة فيها، و اختُبرت فاعلية المستخلص تجاه ثلاث عزلات جرثومية ممرضة Pseudomonas sp., Salmonella sp., Klebsiella sp. بوساطة طريقة مقايسة السمية الخلوية باستعمال اختبار .MTT أظهر المستخلص الأسيتوني فاعلية مضادة للجراثيم المختبرة، و لكن عزلة Salmonella sp. كانت الأكثر حساسية تجاه المستخلص حتى عند التراكيز المنخفضة.
Lichen (Lecanora epibryon) collected from Arshany reservation at Idleb city was taken for screening antibacterial activity. Acetone was used as solvent for extraction of active compounds from lichen. The activity of extract was tested against three isolated infectious bacteria (Pseudomonas sp., Salmonella sp., Klebsiella sp.) by cytotoxicity assay using MTT test. Acetone extract of lichen showed antibacterial activity against all test organisms, but Salmonella sp. was highly susceptible to the extract even at low concentration.
References used
Baytop T. (1999). Therapy with Medicinal plants in Turkey (Past and Present). Istanbul, University, Istanbul, 1-233
Bhattarai H. D., Paudel B., Hong S. G., Lee H. K., Yim J. H. (2008). Thin layer chromatography analysis of antioxidant constituents of lichen from Antractica. J. Nat. Med. 62: 481-484
Cansarana D., Kahya D., Yurdakulol E., Atakol O. (2006). Identification and quantitation of usnic acid from the lichen Usnea species of Anatolia and antimicrobial activity. Z. Naturforsch. C, 61: 773-776
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Inhibition activity of extracts (acetone, ethanol, water) parts of the (Myrtus
communis L.) (root, stem, leaves, fruits) with concentrations (25,50,75,100%)
was carried out against growth of Gr- and Gr+ bacteria which were isolated and
identified
from the pathogenic specimens of The University Children's Hospital
bacterial laboratory in Damascus, using agar well diffusion method on Mueller
– Hinton medium.
Activity of Ziziphora tenuior L. leaves organic extracts against four pathogenic
bacteria, which were isolated from clinical samples and prepared from Al-Assad hospital
laboratory in Lattakia, were tested by disc diffusion method through 2014.
Res
ults showed that Ziziphora tenuior L. leaves extracts have antibacterial activity
against all Gram positive and negative bacteria. Inhibition zones were recorded between
9mm against Pseudomonas aeruginosa and 44mm against Staphylococcus aureus at
concentration of 200mg/ml for the methanolic extract. Whereas the inhibition zones for the
ethanolic extract were recorded between 7mm at concentration of 40mg/ml against
Streptococcus sp. and 34mm at concentration of 200mg/ml against Staphylococcus aureus.
These results represent the importance of Ziziphora tenuior L. as a source of natural
substances counteraction of pathogenic bacteria.
The antibacterial activity of Inula viscosa L. leaves extracts against some pathogenic bacteria, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method.
Results showed that water extracts had antibacte
rial activity against all Gram positive and negative bacteria. The diameters of inhibition zones were between 9 mm against Pseudomonas aeroginosa and 41 mm against Proteus vulgaris.
All Gram positive bacteria showed sensitivity to all organic extracts, so they had antibacterial activity, while Gram negative bacteria showed resistance to chloroform extract except for the one of Klebsiella pneumoniae with an inhibition zone of 12 mm, and resistance for dichlormethan extract except for Pseudomonas aeruginosa with an inhibition zone of 15 mm. there was resistance for diethyl extract except for Serratia marcescens with an inhibition zone of 32 mm.
Ethyl acetate extract had antibacterial activity against all bacteria. Ethanol, methanol and acetone extracts showed antibacterial activity against all bacteria except for Escherichia coli with an inhibition zone of 10 mm.
This study indicated that water and organic Inula viscosa L. leaves extracts had antibacterial activity against pathogenic bacteria which also was tested, especially water extract, ethanol, methanol, ethyl acetate and hexane extracts. So the Inula viscosa L. leaves extracts may be sources for natural antibiotic in future.
Pyocyanin was extracted from cultures of Pseudomonas
aeruginosa, which was isolated from sea water by chloroform.
Results study the effect of pyocyanin against human pathogenic
bacteria, which were isolated from Al-Assad hospital laboratory in
La
ttakia, with disk diffusion method showed that pyocyanin has
antibacterial activity against Gram- positive bacteria.
Four isolates of Streptomyces sp., St.1 and St.2 were isolated
from garden soil of science faculty, St.3 and St.4 were isolated
from Al-Shameia soil Lattakia, by serial dilution on Starch Casein
Agar medium.
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