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In this work we study the kinetic of Catalase and Guaiacol peroxidase and the effect of : pH, temperature, enzyme concentration and substrate concentration on the activity of these enzymes in the mesocotyles of Zea mays ( Ghoto82 ) 6 day old.
In this study a total of /14/ fungal genera have been isolated from seeds; seedlings roots and soilsofCypress and Pine species (brutia; canary and stone) samples brought from Hinnade's nursery, and it were: Alternaria; Rhizoctoni; Fusarium; Chaetomiu m; Mucor; Phythophtora; Rhizopus; Aspergillus; Penicillium; Cladosporium; Saccharomyces; Sordaria; Pytiumand Trichoderma. The first 12 genera were isolated from seeds. Alternaria andFusarium appeared on alldifferent types of plants with the highest frequency of the first genus 23.3% and 36.6% of the second one on cypress. The first 5 genera were isolated from seedlings roots in addition to genus Pythium. And also the Genus Fusariumwas frequented on all study types with high frequencies reached to 58.5%onbrutia.The genusRhizoctoniawas not isolated from seeds and roots of Cypress while it was isolated from seeds and roots of all species of pine./7/ fungal genera were isolated from seedlings soils three of it (Alternaria,Aspergillus,Fusarium) were isolated from the soils of all species of plants with the highest percentages of the first 68.97% on cypress's soil and 22.22% of the second from stone pine and 35.13%of the genusFusarium from brutia. The highest frequency was for genus Fusarium 35.13% on brutia. The genusPythium was onlyisolated from the seeds and soils of brutia pine.
This research aims to study the effects of some oxidoreductase enzymes activity in the seedlings of maize plant stem cells, which Cultured in the present of the following cations: Na+, K+, Mg2+, and Ca2+. (GHOTA 82) yallow corn seeds were used, an d cultured for six days in water and in solution of cations with different concentrations. In brief, preparation and titration processes consist of extraction of enzymatic solutions from plant stem cells seedlings by buffer solutions (at appropriate PH for each enzyme). The results show that the activity of used enzymes increases with the increasing concentrations of the used cations from 1 to 6 g/l by comparison with those of cultured samples in water. Besides the difference in the effect of cations was limited by the concentrations range between 6-9 g/ l.
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