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Isolation and efficacy evaluation of the fungus Beauveria bassiana (Bals.) Vuil. Against larvae and pupae of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

عزل الفطر Beauveria bassiana (Bals.)Vuil. و تقييم فاعليته على يرقات و عذارى دودة ورق القطن Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

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 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




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Six different isolates of the fungus Beauveria bassiana were isolated from local soils, which collected from two different ecosystems (Natural and Cultivated), at the Syrian Coastal region, using the "Galleria Bait Method". The mortality caused by these fungal isolates against Galleria mellonella larvae was 100% after 3- 5 days post treatment. The pathogenicity of these isolates was studied against 3rd instar larvae of Spodoptera littoralis to detect the most virulent isolate. The mortality ranged from 75% to 100% for the all isolates at the 5th day post treatment. Spore suspension of the most virulent isolate was used at concentration 106spore/ml by spraying it on the pupae of S. littoralis. The percentage of adult emergence was 42% in the fungal treatment compared to the control treatment.


Artificial intelligence review:
Research summary
تناولت الدراسة عزل وتقييم فعالية الفطر Beauveria bassiana ضد يرقات وعذارى دودة ورق القطن Spodoptera littoralis. تم عزل ست عزلات من الفطر من ترب محلية في الساحل السوري باستخدام طريقة الطعم Galleria bait method. أظهرت العزلات قدرة إمراضية عالية ضد يرقات دودة الشمع الكبرى Galleria mellonella بنسبة موت 100% بعد 3-5 أيام من المعاملة. تم اختبار فعالية العزلات على يرقات العمر الثالث لدودة ورق القطن، حيث تراوحت نسب الموت بين 75% و100% في اليوم الخامس من المعاملة. استخدم المعلق البوغي للعزلة الأكثر شراسة بتركيز 10^6 بوغ/مل لرش عذارى دودة ورق القطن، وبلغت نسبة خروج البالغات 42% مقارنة مع الشاهد. تشير النتائج إلى فعالية الفطر B. bassiana كعامل مكافحة حيوية ضد دودة ورق القطن، مع توصية بمتابعة البحث عن عزلات محلية أخرى واختبارها على مختلف أطوار الحشرة.
Critical review
دراسة نقدية: الدراسة قدمت نتائج هامة حول فعالية الفطر Beauveria bassiana كمكافحة حيوية لدودة ورق القطن، ولكن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل توسيع نطاق الدراسة ليشمل اختبارات ميدانية لتأكيد فعالية الفطر في الظروف الطبيعية. ثانياً، لم تتناول الدراسة تأثير الفطر على الأنواع غير المستهدفة من الحشرات، وهو جانب مهم لضمان السلامة البيئية. أخيراً، كان من الممكن تقديم تحليل اقتصادي لتكلفة استخدام الفطر مقارنة بالمبيدات الكيميائية التقليدية.
Questions related to the research
  1. ما هي الطريقة المستخدمة لعزل الفطر Beauveria bassiana في الدراسة؟

    تم استخدام طريقة الطعم Galleria bait method لعزل الفطر Beauveria bassiana من الترب المحلية في الساحل السوري.

  2. ما هي نسبة الموت التي حققتها عزلات الفطر على يرقات دودة الشمع الكبرى؟

    حققت عزلات الفطر نسبة موت 100% على يرقات دودة الشمع الكبرى بعد 3-5 أيام من المعاملة.

  3. ما هو التركيز المستخدم للمعلق البوغي للعزلة الأكثر شراسة؟

    تم استخدام تركيز 10^6 بوغ/مل للمعلق البوغي للعزلة الأكثر شراسة لرش عذارى دودة ورق القطن.

  4. ما هي نسبة خروج البالغات من عذارى دودة ورق القطن بعد معالجتها بالفطر؟

    بلغت نسبة خروج البالغات 42% بعد معالجتها بالمعلق البوغي للفطر مقارنة مع الشاهد.


References used
ABBOTT, W.S. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 1, 1925, 265- 267
AHMAD, M., S. Al-MOUGHRABI and A. HAJ HASSAN. Survey of Entomopathogenic Fungi in Soils of Different Ecosystems in Lattakia. Arab Journal of Plant Protection, 29: 2, 2011, 171-178
AHMED, A. M and M. H. EL- KATATNY. Entomopathogenic Fungi As Biopesticides Against The Egyptian Cotton Leaf Worm, Spodoptera Littoralis: Between Biocontrolpromise And Immune-Limitation. J. Egypt. Soc. Toxicol, 37: 1, 2007, 39- 51
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