Do you want to publish a course? Click here

Isolation and efficacy evaluation of the fungus Beauveria bassiana (Bals.) Vuil. Against larvae and pupae of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

عزل الفطر Beauveria bassiana (Bals.)Vuil. و تقييم فاعليته على يرقات و عذارى دودة ورق القطن Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

1404   0   43   0 ( 0 )
 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




Ask ChatGPT about the research

Six different isolates of the fungus Beauveria bassiana were isolated from local soils, which collected from two different ecosystems (Natural and Cultivated), at the Syrian Coastal region, using the "Galleria Bait Method". The mortality caused by these fungal isolates against Galleria mellonella larvae was 100% after 3- 5 days post treatment. The pathogenicity of these isolates was studied against 3rd instar larvae of Spodoptera littoralis to detect the most virulent isolate. The mortality ranged from 75% to 100% for the all isolates at the 5th day post treatment. Spore suspension of the most virulent isolate was used at concentration 106spore/ml by spraying it on the pupae of S. littoralis. The percentage of adult emergence was 42% in the fungal treatment compared to the control treatment.


Artificial intelligence review:
Research summary
تناولت الدراسة عزل وتقييم فعالية الفطر Beauveria bassiana ضد يرقات وعذارى دودة ورق القطن Spodoptera littoralis. تم عزل ست عزلات من الفطر من ترب محلية في الساحل السوري باستخدام طريقة الطعم Galleria bait method. أظهرت العزلات قدرة إمراضية عالية ضد يرقات دودة الشمع الكبرى Galleria mellonella بنسبة موت 100% بعد 3-5 أيام من المعاملة. تم اختبار فعالية العزلات على يرقات العمر الثالث لدودة ورق القطن، حيث تراوحت نسب الموت بين 75% و100% في اليوم الخامس من المعاملة. استخدم المعلق البوغي للعزلة الأكثر شراسة بتركيز 10^6 بوغ/مل لرش عذارى دودة ورق القطن، وبلغت نسبة خروج البالغات 42% مقارنة مع الشاهد. تشير النتائج إلى فعالية الفطر B. bassiana كعامل مكافحة حيوية ضد دودة ورق القطن، مع توصية بمتابعة البحث عن عزلات محلية أخرى واختبارها على مختلف أطوار الحشرة.
Critical review
دراسة نقدية: الدراسة قدمت نتائج هامة حول فعالية الفطر Beauveria bassiana كمكافحة حيوية لدودة ورق القطن، ولكن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل توسيع نطاق الدراسة ليشمل اختبارات ميدانية لتأكيد فعالية الفطر في الظروف الطبيعية. ثانياً، لم تتناول الدراسة تأثير الفطر على الأنواع غير المستهدفة من الحشرات، وهو جانب مهم لضمان السلامة البيئية. أخيراً، كان من الممكن تقديم تحليل اقتصادي لتكلفة استخدام الفطر مقارنة بالمبيدات الكيميائية التقليدية.
Questions related to the research
  1. ما هي الطريقة المستخدمة لعزل الفطر Beauveria bassiana في الدراسة؟

    تم استخدام طريقة الطعم Galleria bait method لعزل الفطر Beauveria bassiana من الترب المحلية في الساحل السوري.

  2. ما هي نسبة الموت التي حققتها عزلات الفطر على يرقات دودة الشمع الكبرى؟

    حققت عزلات الفطر نسبة موت 100% على يرقات دودة الشمع الكبرى بعد 3-5 أيام من المعاملة.

  3. ما هو التركيز المستخدم للمعلق البوغي للعزلة الأكثر شراسة؟

    تم استخدام تركيز 10^6 بوغ/مل للمعلق البوغي للعزلة الأكثر شراسة لرش عذارى دودة ورق القطن.

  4. ما هي نسبة خروج البالغات من عذارى دودة ورق القطن بعد معالجتها بالفطر؟

    بلغت نسبة خروج البالغات 42% بعد معالجتها بالمعلق البوغي للفطر مقارنة مع الشاهد.


References used
ABBOTT, W.S. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 1, 1925, 265- 267
AHMAD, M., S. Al-MOUGHRABI and A. HAJ HASSAN. Survey of Entomopathogenic Fungi in Soils of Different Ecosystems in Lattakia. Arab Journal of Plant Protection, 29: 2, 2011, 171-178
AHMED, A. M and M. H. EL- KATATNY. Entomopathogenic Fungi As Biopesticides Against The Egyptian Cotton Leaf Worm, Spodoptera Littoralis: Between Biocontrolpromise And Immune-Limitation. J. Egypt. Soc. Toxicol, 37: 1, 2007, 39- 51
rate research

Read More

Biological tests were study the pathogenicity of four local isolates of Beauveria bassiana (Bals) orginated from, Latakia , Homs ,ICARDA Jableh on third larval age of Bactrocera oleae.
Gypsy moth (Lymantriadispar) is polyphagous insect .Therefore we had conducted an experiment to determine the influence of the following plant hosts(Malussp, Quercussp ,Prunus. armeniaca, Platanusprientalis, Pinusnigra) onthe growth and development of gypsy moth's larvae. The results showed that duration of larval development was the longest onPinusnigra(77,1±5,46) days and the shortest on Malussp(37,5±4,96) days,for larval growth in length and weight larvae had gained the higher length and weight on Malussp in the order (62±1,9)m.m (2136±9,62) mg. on the other hand the lower length and weight were on Pinusnigra in the order (38±2,44)m.m (545±7,91) m.g. mortality percent of larvae was least on Malussp.and highest on Pinusnigra. Finally, pupae had the best weight with shortest development duration on the Malussp, while the least weight and longest development duration were on the Pinusnigra. There were proportional indirect of larval percentage mortality on relation to plant host, it was highest on lower preference plant.
This study was carried out to determine the efficiency of local Bacillus isolates to control large wax moth larvae (Galleria mellonella L.) at GCSAR laboratory in 2012. Infected larvae were collected from stored wax combs, and the bacteria Bacillus genus were isolated from dead larvae, or that showing disease symptoms of black brown spots on larvae cuticle. Bacterium was grown on T3 medium and identified according to biochemical tests, and the efficacy of isolates was determined on pure colonies of larvae. The results of biochemical tests showed that the isolates belong to Bacillus thuringiensis. Isolates were different in pathogenicity. Bt5 isolate was the most efficient to kill the larvae of large wax moth (72.4 %), and significantly superior all other isolates (p ≤ 0.01). Bt1 isolate showed a significant difference with control but non-significant difference with Bt2, Bt3, Bt4, Bt6 and Bt7 isolates.
Samples from carrot and lettuce roots, sclerotia of Sclerotinia sclerotiorum, and soil were collected from south of Damascus during 2001. 82 isolates were isolated, 48 of them were isolated from roots, 27 from sarfuce of sclerotia, and 7 from soil around roots. Antgonisim capacity of these isolates was tested against S. Sclerotiorum in vitro. 7 bacterial isolates showed antagonistic effect on the growth of S. Sclerotiorum, the antagonism distance ranged bettwen (1.3 – 11 mm). The Isolate (K82.4), which was isolated from soil, showed antagonistic distance (11mm), and was the best of bacterial isolates. Three isolates were iddentified (K82.4 isolated from lettuce soil, FN3 isolated from surface of sclerotia, K4.4 isolated from carrot roots), that belong to Bacillus spp.
comments
Fetching comments Fetching comments
Sign in to be able to follow your search criteria
mircosoft-partner

هل ترغب بارسال اشعارات عن اخر التحديثات في شمرا-اكاديميا