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Register a new userStudy of Ki67 Expression in different histological grades of oral epithelial dysplasia
دراسة تعبيريّة Ki67 في الدرجات النسيجية المختلفة من سوء التصنع البشروي الفموي
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Aِl-Baath University ورقة بحثية
Publication date
2018
fields
Dentistry
and research's language is
العربية
Created by
Shamra Editor
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الهدف من البحث : إثبات وجود و توضع و نمط التكاثر الخلوي في الدرجات النسيجية المختلفة لسوء التصنع البشروي الفموي و في البشرة الفموية الطبيعية بإستخدام الجسم المضاد لبروتين Ki67
Aim of the study: To demonstrate the presence, location and pattern of cell proliferation in different histological grades of oral epithelial dysplasia and normal oral epithelium (NOE) using an antibody directed against the Ki-67 antigen.
References used
Pardee AB. G1 events and regulation of cell proliferation. Science. 1989;246(4930):603-8
Tumuluri V, Thomas G, Fraser I. Analysis of the Ki‐67 antigen at the invasive tumour front of human oral squamous cell carcinoma. Journal of oral pathology & medicine. 2002;31(10):598-604
Bacchi C, Gown A. Detection of cell proliferation in tissue sections. Brazilian journal of medical and biological research= Revista brasileira de pesquisas medicas e biologicas. 1993;26(7):677-87
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Oral epithelial dysplasia is a precancerous change develops in the oral mucosa, and
leukoplakia is the most common precancerous lesions in oral mucosa.
The epithelial-mesenchymal transition (EMT) is considered the mechanism through
which the oral
leukoplakia becomes malignant, and it's also responsible for the invasion
and metastasis of cancers. This study investigates the expression of β-catenin in epithelialmesenchymal
transition detected by immunohistochemical staining in the different grades
of oral epithelial dysplasia.
The sample consisted of 45 formalin-fixed, paraffin-embedded specimens of oral
leukoplakia with different grades of oral epithelial dysplasia; specimenswere
immunohistochemically stained with β-catenin antibody.
The control group was consisted of biopsies from the normal oral epithelia.
The EMT rates are associated with the severity of the oral epithelial dysplasia.That
means, the epithelial-mesenchymal transition marker β-catenin could be used to evaluate
the potentiality of malignant transformation in the precancerous lesions.
Background and Objective : Bone morphogenic protine-2 (BMP-2) plays an essential
role in mesenchymal cells differentiation into osteoblasts، through many intracellular
pathways which crosses with tumoral pathways . Oral squamous cell carcinoma is a
n
invasive epithelial neoplasm، counts more than 90% of head and neck malignancies. It is
classified into three degrees according to the epithelial cells differentiation. The study
aimed to investigate the changes in BMP-2 expression according to the histological
degrees of the tumor.
Materials and methods : In the present study، BMP-2 expression was compared
immunohistochemically among oral squamous cell carcinoma in 27 cases (19 male+8
female) ranging between (22-74) years، mean age of (48) years. Patients have been divided
into 3 groups (each contain 9 case) according to the histological grade.
Results : By analyzing the statistical results, No significant correlation between
BMP-2 expression and the histological grad was observed. The severity of expression was
not affected by the tumour degree. Changes in membranous localization and intense
cytoplasmic staining were also not observed.
Conclusion : according to this study BMP-2 does not represent a prognostic marker
for the histological grade of oral squamous cell carcinoma.
Background & Objective: This immunohistochemical study is to investigate the correlation between p53 & Ki67 and the grade of malignancy of squamous cell carcinoma, to estimate functional behavior of the cancerous cell by Ck17, and to compare the resu
lts with the histological grading.
Methods & Materials: 64 samples (biopsies) were chosen to check for p53, Ki67 & Ck17 at department of oral histology and pathology between 2002- 2009.
Conclusion: There is a strong positive correlation between the degree of malignancy and the signs of cellular atypism (morphological changes) and the immunhistochemical positivity specially p53 and Ki67, and there is a strong negative correlation between the positivity of p53, Ki67 and the plasmolymphatic
infiltrate. We must be sure of the correct diagnosis of squamous cell carcinoma by histological and immunohistochemical examination, and attention should be given to use more than one immune reactive.
This retrospective study is to correlate oral squamous cell carcinoma with age,
gender and location of lesion, and to investigate the correlation between the grade of the malignancy and
lymph nodes in patients of the faculty of dentistry, Damascus university.
Smoking tobacco is probably the single most significant source of
toxic chemical exposure to humans, and that affects many human
organs .smoking is a major risk factor for various types of
cancers.The specimenen compassed of two groups of voluntee
rs:
the group included 20 volunteers of smokers, the second
group included14 volunteers of non-smokers as a control group.
Surgical biopsy from oral buccal mucosa were taken and stained
with ( P21,p27 clone) antibody by IHC, and we count the positive
stained cells in two study groups to show if the gene p21,p27 is
affected by cigarette smoking, and investigate the possibility of a
mutation in it .statistical clear differences between the two groups .
the control group is negative while the two groups was positive.
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