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Register a new userInvestigation the expression of β-catenin in oral epithelial dysplasia
تحرّي تعبيرية بروتين الـβ-Catenin في سوء التصنّع البشروي الفموي
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Tishreen University ورقة بحثية
Publication date
2015
fields
Dentistry
and research's language is
العربية
Created by
Shamra Editor
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تعتبر آفات سوء التصنّع الفمويّة في كثير من حالاتها آفات ما قبل سرطانيّة، و تقوم الخلايا الظهاريّة السرطانية بالغزو و الاستعمار من خلال آليّة التحوّل الظهاري الميزانشيمي. و قد أجريت هذه الدراسة لتحرّي التحوّل الظهاري الميزانشيمي عبر دراسة تغيرات الـβ-catenin في الدرجات المختلفة لسوء التصنّع البشروي الفموي. تمّ دراسة 45 خزعة مثبّتة بالفورمالين و مدمجة بشمع البارافين مؤرشفة و مشخّصة على أنّها أفات ذات سوء تصنّع بشروي فموي، و تمّ تقطيعها و تلوينها بالـ H&E ثمّ تقييم درجة سوء تصنّعها و تقسيمها لثلاث مجموعات حسب درجة سوء التصنّع، ثمّ لوِّنت بالملوّن المناعي الكيميانسيجي β-catenin لدراسة التحول الظهاري الميزانشيمي فيها. و تم مقارنتها مع عينة شاهدة من نسج فموية سليمة. و استنتج أنهيزداد معدّل التحوّل الظهاري الميزانشيمي مع زيادة درجات سوء التصنّع. و يزداد معدّل التطفُّر في البروتينات التي تضبط مستويات الـ β-cateninمع زيادة درجات سوء التصنّع. فبالتالي يمكن استخدام الملون المناعي الكيميانسيجي الـ β-catenin للتنبّؤ باحتماليّة التحوّل الخبيث للآفات الفمويّة البشرويّة.
Oral epithelial dysplasia is a precancerous change develops in the oral mucosa, and leukoplakia is the most common precancerous lesions in oral mucosa. The epithelial-mesenchymal transition (EMT) is considered the mechanism through which the oral leukoplakia becomes malignant, and it's also responsible for the invasion and metastasis of cancers. This study investigates the expression of β-catenin in epithelialmesenchymal transition detected by immunohistochemical staining in the different grades of oral epithelial dysplasia. The sample consisted of 45 formalin-fixed, paraffin-embedded specimens of oral leukoplakia with different grades of oral epithelial dysplasia; specimenswere immunohistochemically stained with β-catenin antibody. The control group was consisted of biopsies from the normal oral epithelia. The EMT rates are associated with the severity of the oral epithelial dysplasia.That means, the epithelial-mesenchymal transition marker β-catenin could be used to evaluate the potentiality of malignant transformation in the precancerous lesions.
References used
ABBEY, L. M., KAUGARS, G. E., GUNSOLLEY, J. C., BURNS, J. C., PAGE, D. G., SVIRSKY, J. A., EISENBERG, E., KRUTCHKOFF, D. J. & CUSHING, M. Intraexaminer and interexaminer reliability in the diagnosis of oral epithelial dysplasia. Oral Surg Oral Med Oral Pathol Oral Radiol Endod,1995. 80, 188-91
ACLOQUE, H., ADAMS, M. S., FISHWICK, K., BRONNER-FRASER, M. & NIETO, M. A.. Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. J Clin Invest,2009119, 1438-49
BARAKAT, S.. Histologic, Histochemic and Immunohistochemic comparative study between Normal, Precancerous and Cancerous oral mucosa. PHD Research in Dentistry Faculty, Damascus University.2005
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epithelial dysplasia and normal oral epithelium (NOE) using an
antibody directed against the Ki-67 antigen.
Recombinant human bone morphogenetic protein-2 (rhBMP-2 ) is
effectively to regenerate defects of the maxillary and mandible
bones. Such defects are sometimes caused by resection of oral
squamous cell carcinoma (OSCC) yet the biologic effects of
rhBMP-2 on this malignancy and its histological relations and the
ability of invasion and metastasis are not fully clear.
Aim of the study : this study aimed to investigate the effects of
rhBMP2 on E-cadherin expression , which plays a pivotal role in the
cellular adhesion through the adherent junctions.
Aim of study: study of the differences in the protein expression of
Cyclin-dependent Kinase CdK1, CdK2 in Oral Squamous Cell
Carcinoma (OSCC) and in clinically- normal oral mucosa.
Smoking tobacco is probably the single most significant source of
toxic chemical exposure to humans, and that affects many human
organs .smoking is a major risk factor for various types of
cancers.The specimenen compassed of two groups of voluntee
rs:
the group included 20 volunteers of smokers, the second
group included14 volunteers of non-smokers as a control group.
Surgical biopsy from oral buccal mucosa were taken and stained
with ( P21,p27 clone) antibody by IHC, and we count the positive
stained cells in two study groups to show if the gene p21,p27 is
affected by cigarette smoking, and investigate the possibility of a
mutation in it .statistical clear differences between the two groups .
the control group is negative while the two groups was positive.
Background and Objective : Bone morphogenic protine-2 (BMP-2) plays an essential
role in mesenchymal cells differentiation into osteoblasts، through many intracellular
pathways which crosses with tumoral pathways . Oral squamous cell carcinoma is a
n
invasive epithelial neoplasm، counts more than 90% of head and neck malignancies. It is
classified into three degrees according to the epithelial cells differentiation. The study
aimed to investigate the changes in BMP-2 expression according to the histological
degrees of the tumor.
Materials and methods : In the present study، BMP-2 expression was compared
immunohistochemically among oral squamous cell carcinoma in 27 cases (19 male+8
female) ranging between (22-74) years، mean age of (48) years. Patients have been divided
into 3 groups (each contain 9 case) according to the histological grade.
Results : By analyzing the statistical results, No significant correlation between
BMP-2 expression and the histological grad was observed. The severity of expression was
not affected by the tumour degree. Changes in membranous localization and intense
cytoplasmic staining were also not observed.
Conclusion : according to this study BMP-2 does not represent a prognostic marker
for the histological grade of oral squamous cell carcinoma.
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