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Study of the protective and therapeutic effects of the Ecballium Elaterium ʺfruit juiceʺ on experimental infection with Eimeria Stiedae in Domestic Rabbits

دراسة التأثير الوقائي و العلاجي لعصارة ثمرة الإكباليوم (قثاء الحمار) على الأرانب المصابة تجربياً بكوكسيديا ستيدي

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 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




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A study was conducted to investigate the protective and therapeutic effects of Ecballium elaterium juice against experimental infection with Eimeria stiedae in rabbits. Thirty- two rabbits were divided into four groups (eight rabbits in each group) : healthy control group (HC); challenged – Ecballium elaterium –protected group (CEEP) which received a daily dose of (45) μl\kg body weight Ecballium elaterium juice intra-peritoneal for five days before challenged with E. stiedae ; challenged – Ecballium elaterium –treated group (CEET) which received a daily dose of (45)μl\kg body weight Ecballium elaterium juice intra-peritoneal for five days after challenged with of E .stiedae and infected group (IC).The challenge dose was 40000 sporulated E. stiedae oocyst per rabbits.

References used
Abbas, S.M (2009): Field study of some endoparasites in local rabbits. Proceeding of the ninth veterinary scientific conference. 1:157-160
Abdel-Mageed, k.N.; Abu El-Ezz, N.M.& Abdel Rahman, E.H. (2005): Protective effect of Eimeria stiedae corporantigen against hepatic coccidiosis in rabbits. J. Egypt. Soc. Parasitol., 35(2): 581-595
Abed, H.H. & Yakoob, A.Y (2013):study the protective and therapeutic effects of crude garlic on mortality, oocyst output and hepatic lesions in experimental infection with eimeria stiedae in domestic rabbits.Bas .j. vet. Res. Vol.12,No.2
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The aim of this experiment was to study the protective and therapeutic effects of Ecballium elaterium juice against experimental infection with Eimeria stiedae in rabbits. thirty- two rabbits Divided into four groups (eight rabbits in each group) : healthy control group; challenged –Ecballium elaterium –protected group, which received a daily dose of (45)μl\kg body weight Ecballium elaterium juice intra-peritoneal for five days before challenged with E. stiedae; challenged –Ecballium elaterium – treated group which received a daily dose of (45)μl\kg body weight Ecballium elaterium juice intra-peritoneal for five days after challenged with of E .stiedae and infected group. The challenge dose was 40000 sporulated E. stiedae oocyst per rabbit.
The aim of this experiment to study some of the blood parameters associated with hepatic coccidiosis induced by Eimeria stiedae in rabbits, and compare it with the blood changes when infected rabbits treating with the toltrazuril compound. 21 rab bit-old (4-8 weeks) were Used for this experiment of both sexes and were divided into three groups (seven rabbits in each group): Group of natural witness, and a Group of patient witness, where the infection caused by challenge dose of about forty thousand mature oocyst for each rabbit by mouth, and a treatment group that has been given the same previous challenge dose.
The aim of this experiment was to study Histological changes associated with different stages of infection with hepatic coccidiosis induced by Eimeria stiedae in rabbits.
This study aims to provide GC-FID and GC-MS analyses of the essential oil of dried aerial parts of Ecballium elaterium L. grown in Jordan and examining its cytotoxicity capacity. Methods: Essential oil was obtained by hydrodistillation using Clevenge r apparatus. MTT assay method was used to investigate the plant's in vitro antiproliferative activity against MCF-7, Caco-2 and Panc-1 cancer cell lines in addition to normal fibroblast cells. Results: E. elaterium hydrodistilled oil yielded thirty one components, accounting for 76.3% of the total oil content. High contents of nonterpenoidal compounds, sesquiterpenes, and monoterpene characterized the volatile fractions with hinesol (17.2%), the principal compound, benzaldehyde (12.3%) and E-β-ionone (7.8%) as the major constituents. E. elaterium ethanolic extract showed good activity against MCF-7 and Caco-2 cells (IC50 values=29.67 μg/mL and 17.64 μg/mL, respectively). Moreover, all extracts were safe on normal human cells. In conclusion: Evaluation of E. elaterium volatile oil has been conducted for the first time in Jordan; also various extracts were tested for the first time against Panc-1 cells. Furthermore, based on the obtained results, ethanol extract of E. elaterium may be advocated as candidate for breast and colorectal cancers management.
This study is aimed to evaluate the role of Platelet Rich Plasma PRP on osteogensis in periosteal distraction. The sample consisted of 12 rabbits from local spices. The sample divided equally into two groups, titanium mesh with holes was used in to elevate the periosteum and the space between the surface of bone and the mesh was filled with collagen sponge, in the test group the sponge was injected with PRP. The rabbits were killed after eight weeks after operation and the samples were prepared for histological study. The histological study of specimens of control group showed formation of small island of osteoid with large areas of connective tissue. While, in test group there was an increasing in bone formation in form of wide area of woven bone and decreasing in connective tissue. We conclude that application of Platelet Rich Plasma PRP lead to improvement in bone formation in periosteal distraction.
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