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Ortho-phenylene diamine Study of kinetic by catalase

دراسة حركية أورثو- فينيلين ثنائي الأمين بإنزيم الكاتالاز

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 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




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Catalase enzyme has two functions, first is catalytic and destroyed with high temperature. The second function oxidic , increasing with high temperature. The catalase enzyme effects on the substrate of ortho – phenylene diamine turning it into aromatic cancerogenus azo compounds organic acids such as, benzoic acid, salicylic acid and ascorbic acid inhibit the effect of catalase enzyme substrate ortho – phenylene diamine. The inhibition is competitive when the benzoic acid and salicylic acid effects , while the inhibition is non competitive when the ascorbic acid effect.


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Research summary
يتناول البحث دراسة حركية إنزيم الكاتالاز وتأثيره على مادة أورثو-فينيلين ثنائي الأمين (OPDA)، حيث يحولها إلى مركبات آزو عطرية مسرطنة. يوضح البحث أن إنزيم الكاتالاز يمتلك وظيفتين: الأولى تحفيزية تتحلل بارتفاع درجة الحرارة، والثانية أوكسيدية يزداد نشاطها بارتفاع درجة الحرارة. كما يدرس البحث تأثير الحموض العضوية مثل حمض البنزوئيك، حمض الساليسليك، وحمض الأسكوربيك على تثبيط فعل إنزيم الكاتالاز، حيث يكون التثبيط تنافسيًا بوجود حمضي البنزوئيك والساليسليك، ولا تنافسيًا بوجود حمض الأسكوربيك. يهدف البحث إلى دراسة تأثير تركيز الإنزيم، الرقم الهيدروجيني، ودرجة الحرارة على سرعة التفاعل الإنزيمي، بالإضافة إلى تأثير تراكيز المثبطات على سرعة التفاعل. تم إجراء التجارب باستخدام التحليل الطيفي UV لتحديد تركيزات الإنزيم والمثبطات، وتم تحليل النتائج لتحديد الثوابت الحركية المختلفة.
Questions related to the research
  1. ما هي الوظائف الرئيسية لإنزيم الكاتالاز؟

    يمتلك إنزيم الكاتالاز وظيفتين رئيسيتين: الأولى تحفيزية تتحلل بارتفاع درجة الحرارة، والثانية أوكسيدية يزداد نشاطها بارتفاع درجة الحرارة.

  2. كيف يؤثر إنزيم الكاتالاز على مادة أورثو-فينيلين ثنائي الأمين؟

    يحول إنزيم الكاتالاز مادة أورثو-فينيلين ثنائي الأمين إلى مركبات آزو عطرية مسرطنة.

  3. ما هو نوع التثبيط الذي يحدث بوجود حمض الأسكوربيك؟

    يكون التثبيط لا تنافسيًا بوجود حمض الأسكوربيك.

  4. ما هي العوامل التي تم دراستها لتحديد تأثيرها على سرعة التفاعل الإنزيمي؟

    تم دراسة تأثير تركيز الإنزيم، الرقم الهيدروجيني، ودرجة الحرارة على سرعة التفاعل الإنزيمي.


References used
Seince E, F, and Knyazov P,.G , 1986-Molecular Oncologia. Leningrad,Medicine p.349
Kinzel ,V , Scribner J,.D , 1977- Cancer Experiments and Concepts . MIR, Moscow, p.360
Dixon, M, Webb, E .C. 1982- Enzymes . MIR , Moscow,p.1115
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