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Register a new userThe pathogenicity of local isolates of Beauveria bassiana (Balsmo) Vuill for larvae of Bactrocera oleae ( Rossi. 1790)
القدرة الإمراضية لعزلات محلية من الفطر Beauveria bassiana (Balsmo) Vuill على يرقات ذبابة ثمار الزيتون (Bactrocera oleae (Rossi. 1790
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أجريت الاختبارات الحيوية لدراسة القدرة الامراضية لأربع عزلات محلية من الفطر Beauveria bassiana مأخوذة من اللاذقية, حمص, ايكاردا حلب, جبلة على يرقات العمر الثالث لذبابة ثمار الزيتون Bactrocera oleae.
Biological tests were study the pathogenicity of four local isolates of Beauveria bassiana (Bals) orginated from, Latakia , Homs ,ICARDA Jableh on third larval age of Bactrocera oleae.
References used
ABBOTT,W.S.1925.A method of computing the effectiveness of insecticide. Journal of Ecocncmic Entomology,Vol.18,N.2,265-267
BARSON, G., N. Renn, and A. F. Bywater. 1994.Laboratory evaluation of six species of entomopathogenic fungi for the control of the house ßy (Musca domesticaL.) a pest of intensive animal units. J. Invertebr. Pathol. 64: 107Ð113
ALUJA, M. 1993.Manejo Integrado de la mosca de la fruta Trillas, Mexico D.F. Aluja, M. 1994. Bionomics and .management ofAnastrepha Annu. Rev. Entomol. 39: 155Ð178
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The pathogenicity of three isolates of the fungus Beauveria
bassiana (Bals.) Vuill were evaluated on potato tuber moth
Phthorimaea operculella (Zeller) (Lep.,Gelechiidae).
Six different isolates of the fungus Beauveria bassiana were isolated from local
soils, which collected from two different ecosystems (Natural and Cultivated), at the
Syrian Coastal region, using the "Galleria Bait Method". The mortality caused by
these
fungal isolates against Galleria mellonella larvae was 100% after 3- 5 days post
treatment. The pathogenicity of these isolates was studied against 3rd instar larvae of
Spodoptera littoralis to detect the most virulent isolate. The mortality ranged from 75% to
100% for the all isolates at the 5th day post treatment. Spore suspension of the most
virulent isolate was used at concentration 106spore/ml by spraying it on the pupae of S.
littoralis. The percentage of adult emergence was 42% in the fungal treatment compared to
the control treatment.
تم في هذا البحث دراسة تأثير تغذية يرقات العمرين الأول و الرابع لفراشة درنات البطاطا P. operculella على نباتات بطاطا ملقحة بالمعلق البوغي للفطر B.bassiana تركيزه (0.75×10^7 بوغة\مل).
Forty Isolates of Streptomyces bacteria were obtained from soil samples
from different regions of Syria (Damascus, Sweida, Daraa, Homs, Latakia,
Damascus, Hasakeh, Deir-ezzor) during 2012. The isolates were identified using
ISP (International Stre
ptomyces Project) methodology based on morphological
and physiological criteria. Isolates were grown on selective solid media
depending on growth density as an indicator of the consumption of xylose as an
unique carbon source then on liquid media to determine their ability to
produce the glucose isomerase enzyme. The crude enzyme was extracted using
CTAB 0.1% solution, the enzyme activity was estimated using glucose as a
substrate and the end product was measured colorimetrically at 560 nm.
Results showed that the 40 isolates belong to 29 different species of
Streptomyces. All isolates were able to produce glucose isomerase but they
varied in their efficiency. Isolate SH10 (S. roseiscleroticus) was distinguished by
its high level of enzyme production reaching 4.9 units /ml.
This study was carried out to determine the efficiency of local Bacillus isolates to
control large wax moth larvae (Galleria mellonella L.) at GCSAR laboratory in 2012.
Infected larvae were collected from stored wax combs, and the bacteria Bacillus
genus were isolated from dead larvae, or that showing disease symptoms of black
brown spots on larvae cuticle. Bacterium was grown on T3 medium and identified
according to biochemical tests, and the efficacy of isolates was determined on pure
colonies of larvae. The results of biochemical tests showed that the isolates belong
to Bacillus thuringiensis. Isolates were different in pathogenicity. Bt5 isolate was
the most efficient to kill the larvae of large wax moth (72.4 %), and significantly
superior all other isolates (p ≤ 0.01). Bt1 isolate showed a significant difference
with control but non-significant difference with Bt2, Bt3, Bt4, Bt6 and Bt7 isolates.
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