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Register a new userDirect Diagnosis of Cutaneous Leishmaniasis :What Influence the Sensitivity of Dermal Scrapping
التشخيص المباشر لليشمانيا الجلدية: ما الذي يؤثر على حساسية الكشاطة الجلدية
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Aِl-Baath University ورقة بحثية
Publication date
2017
fields
Medicine
and research's language is
العربية
Authors
أمال عساف( باحث )
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Shamra Editor
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ماتزال الليشمانيا الجلدية تمثل أحد اهم المشاكل الصحية في سوريا التي شهدت ازديادا كبيرا بعدد الغصابات المسجلة خاصة في ظل الأزمة, ما يزال الفحص المجهري للكشاطة الجلدية الملونة بغيمزا الطريقة المناسبة لتشخيص الليشمانيا بسبب سهولة إجرائها, سرعة تحضيرها, و عدم حاجتها لتجهيزات مخبرية معقدة.
Cutaneous leishmaniasis is still one of the most important health problems in Syria which witnessed increasing the numbers of cutnaneous leishmaniasis reported, especially during the crisis ,Also the direct microscopic examination of Giemsa-stained scrapings of lesions is still a suitable method for diagnosis of cutaneous leishmaniasis due to the low cost, ease of performance, speed, and lack of a need for sophisticated laboratory equipment.
References used
Manual for case management of cutaneous leishmaniasis in the WHO Eastern Mediterranean 2014. WHO Regional Publications, Eastern Mediterranean Series; 35
Jose A. Ruiz Postigo 2010.Leishmaniasis in the World Health Organization Eastern Mediterranean Region. International Journal of Antimicrobial Agents, 36S S62-S65
Desjeux P 2004. Leishmaniasis current situation and new perspectives. Copm Immunol Microbiol Infect Dis 27(5):305-323
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Cutaneous Leishmaniasis (CL) is one of the most prevalent
clinical forms of leishmaniasis , also it is endemic disease in Syria. And in
spite of the great efforts to control the disease the annual incidence is still
increased. Furthermore, tourism
, migration, and prevalence of acquired
immunodeficiency (AIDS) all these factors increase the spread of this
disease to new areas around the world.
Recently, studies suggest that cytokines gene polymorphisms can contribute
to resistance or susceptibility to many diseases and one of these diseases is
CL .
In this study the total of ٤١٥ cases of cutaneous leishmaniasis was evaluated
from Dermatology hospital and General clinic in Damascus from February
٢٠٠١ to February ٢٠٠٢. Most of these cases was reported in Damascus and its
suburb, and when we ma
de the direct microscopic test, amastigotes were
detectable in ٢٧١ cases, and the positive cases was increased to ٣٦٤ cases by
realizing the parasite culture into diphasic N. N. N. media, which confirm the
diagnosis.
This study was performed on blood samples of 60 people [21females, 39
males]which have visited the center of leishmaniasis in lattakia city, and their infection
with cutaneous leishmaniasis was confirmed. The people were divided into three age
gro
ups where each group contained 20 patients, the first group [10-18 years,3females and
17males], the second group [19-30 years,8 females,12males],and the third group [31-
50years,10 females and 10males]. The following tests were evaluated to each blood
sample: total white blood cell count, differential white blood cells count, hematocrit test,
and blood group type test.
According to the high specificity and sensitivity of ELISA test, we tried in
this preliminary study, to certify the usefulness of ELISA test in the detection of
specific antibodies (IgG) to cutaneous leishmaniasis(CL) in SYRIA, in order to
certify
the diagnosis of this disease by using the classic methods (observation
microscopic – culture).
coma and patients in the medical and surgical intensive care units, in addition
to trauma and burns patients. These problems are difficult to treat.
This medical problem leads to the deterioration of patients' medical condition and increases the su
ffering
of patients and families. It will lead to continuous draining of their financial and moral resources.
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