Do you want to publish a course? Click here

Effect of Leishmania Parasite Infection in Some Blood Parameters of People Infected with Cutaneous Leishmaniasis in Syria

تأثير الخمج بطفيلي الليشمانيا في بعض معايير الدم عند الأشخاص المصابين بداء الليشمانيا الجلدية في سورية

1477   0   24   0 ( 0 )
 Publication date 2015
  fields Biology
and research's language is العربية
 Created by Shamra Editor




Ask ChatGPT about the research

This study was performed on blood samples of 60 people [21females, 39 males]which have visited the center of leishmaniasis in lattakia city, and their infection with cutaneous leishmaniasis was confirmed. The people were divided into three age groups where each group contained 20 patients, the first group [10-18 years,3females and 17males], the second group [19-30 years,8 females,12males],and the third group [31- 50years,10 females and 10males]. The following tests were evaluated to each blood sample: total white blood cell count, differential white blood cells count, hematocrit test, and blood group type test.


Artificial intelligence review:
Research summary
أجريت هذه الدراسة على 60 مريضًا مصابين بداء الليشمانيا الجلدية في مدينة اللاذقية بسوريا، حيث تم تقسيمهم إلى ثلاث مجموعات عمرية. تم تحليل عينات الدم لتحديد التعداد الكلي لخلايا الدم البيض، العد التمييزي لخلايا الدم البيض، قيمة الهيماتوكريت، ونوع الزمرة الدموية. أظهرت النتائج ارتفاعًا معنويًا في نسبة الحمضات واللمفاويات، وانخفاضًا معنويًا في التعداد الكلي لخلايا الدم البيض، العدلات، الوحيدات، وقيمة الهيماتوكريت. لم تُظهر الدراسة فروقًا معنوية بين الفئات العمرية الثلاث ولم تجد علاقة ارتباط معنوي بين أي من المعايير المذكورة وعمر الخمج بالليشمانيا الجلدية. كما أظهرت توافقًا في توزع الزمر الدموية مع نسبة توزعها في سوريا.
Critical review
دراسة نقدية: تعتبر هذه الدراسة خطوة مهمة في فهم تأثير داء الليشمانيا الجلدية على معايير الدم في المرضى السوريين. ومع ذلك، يمكن تحسين الدراسة من خلال زيادة حجم العينة لتشمل مناطق جغرافية أخرى في سوريا، مما قد يوفر نتائج أكثر شمولية. كما يمكن أن تكون هناك حاجة إلى دراسات إضافية لفهم الآليات البيولوجية التي تؤدي إلى التغيرات في معايير الدم. وأخيرًا، يمكن أن تكون هناك حاجة إلى استخدام تقنيات تحليلية أكثر تقدمًا مثل التحليل الجيني لفهم تأثيرات الطفيلي على المستوى الجزيئي.
Questions related to the research
  1. ما هي الفئات العمرية التي شملتها الدراسة؟

    شملت الدراسة ثلاث فئات عمرية: الأولى (10-18 عامًا)، الثانية (19-30 عامًا)، والثالثة (31-50 عامًا).

  2. ما هي التغيرات الرئيسية التي لوحظت في معايير الدم؟

    لوحظ ارتفاع معنوي في نسبة الحمضات واللمفاويات، وانخفاض معنوي في التعداد الكلي لخلايا الدم البيض، العدلات، الوحيدات، وقيمة الهيماتوكريت.

  3. هل وجدت الدراسة علاقة بين نوع الزمرة الدموية والخمج بالليشمانيا الجلدية؟

    لم تجد الدراسة علاقة ارتباط معنوي بين نوع الزمرة الدموية والخمج بالليشمانيا الجلدية.

  4. ما هي أهمية هذه الدراسة؟

    تكمن أهمية الدراسة في استقصاء احتمال وجود علاقة بين الخمج بالليشمانيا الجلدية وتغيرات معايير الدم بما يخدم تشخيص مثل هذه الحالات مخبريًا والتعرف إلى الأمراض التي يمكن أن تُحدث تغيرًا في نسب خلايا الدم البيض.


References used
World Health Organization. Consultative Meeting of Cutaneous Leishmaniasis. geneva. 2007
SHARMA, U; SINGH, S. Immunobiology Of Leishmaniasis. Indian Journal Experimental Biology, India,Vol.47, 2009, 412-423
AWASTHI, A, MATHUR, K, SAHA, B. Immune response to Leishmania infection. Indian J Med Res, Vol.119, 2004, 238-258
rate research

Read More

The present study was performed on blood samples belong to 15 patients( 12 males,3 females) infected with cutaneous leishmaniasis, and their healing was also proved after submitting local treatment. Study tests were performed on 2 stages, the first after infection, and the second two months at least after healing. Every patient was subject of the following tests: total, and differential white blood cells count and hematocrit value. In addition ,a study of the morphological changes of white blood cells was conducted.
Through this research, case studies were reviewed which reviewed the dermatology department at Tishreen University Hospital in Lattakia and specialized centers for leishmaniasis and parasitic diseases in Lattakia and Tartous governorates from the beg inning of April 2018 until the end of December 2019. Where Syria is one of the areas endemic with cutaneous leishmaniasis, as we have noticed in recent years the number of skin infections has increased dramatically in all Syrian governorates, which has made leishmaniasis an important health problem that warrants study. In order to isolate and determine the type and strains of the parasite responsible for skin injuries in Syria, in order to develop a plan to combat this disease and limit its spread. Microscopy and transplantation methods are the gold standard for diagnosing cutaneous leishmaniasis, especially in poor areas where this disease is common. Through this study it was found that the age group most exposed to infection is between 16 - 40 years and that the highest incidence was among males. As for the distribution of skin lesions, the highest percentage was recorded on the face, upper and lower extremities.
The parasitic antigens are of great interest in immune responses and in the strategies for developing vaccines. The aim of this study was to identify the major antigens which are expressed in the two leishmania tropica stages, promastigote & amast igote, employing the western blot analysis using the sera collected from mice immunized with the above mentioned parasite stages' total protein.
For studying the effect of cadmium sulphate on some physiological components of blood in male white rabbits. we used (12) animals divided in to two groups (6 animals per group).the experimental one became aqueous cadmium sulphate (5 mg/kg) orally, while the control became water for 28 days (4 weeks). We reported significant decrease (p<0.01) in the count of red blood cells , Hematocrit values and Hemoglobin concentration.
Leishmaniasis spreads in eighty-eight countries, including the Syrian Arab Republic. This disease is caused by a protozoic parasite of the genus Leishmania. Twenty-two species of leishmania were reported to be pathogenic for human. The disease is presented in three clinical forms: cutaneous, mucocutaneous and visceral leishmaniasis. A set of methods for diagnosis and identifying the type of parasite have been developed in order to give appropriate treatment. These methods include isoenzyme analysis, serological and immunological methods and DNA hybridization. The polymerase chain reaction (PCR), using genomic or kinetoplastid DNA; provides an excellent tool for diagnosis and characterization of leishmania species. In this work, the two types of DNA were extracted in one step from cultured isolates taken from patients. We have used kinetoplastid DNA and specific primers to determine parasite species by PCR. These primers amplify species specific fragment. In this study, we have determined that the causative species of the cutaneous leishmaniasis in all patients was L.tropica.
comments
Fetching comments Fetching comments
Sign in to be able to follow your search criteria
mircosoft-partner

هل ترغب بارسال اشعارات عن اخر التحديثات في شمرا-اكاديميا