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Register a new userHistochemical study for detection of localized carbohydrate in Bothriocephalus acheilognathi (Cestoda:Pseudophyllidea)
دراسة كيميائية نسيجية عن تموضع الكربوهيدرات في الدودة Bothriocephalus acheilognathi الشريطية (Cestoda:Pseudophyllidea)
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تـضمن البحـث الحـالي دراسـة كيميائيـة نـسيجية للـدودة الـشريطية Bothriocephalus acheilognathi بهدف تحديد تموضع المواد الكربوهيدراتية (السكرية) في الأنسجة و الأعضاء المختلفة للشريطية من خلال استخدام تقانات حامض البريوديك – شيف PAS و أزرق التولودين و أزرق الالـشيان عند درجة الحموضة (5.2, 1 PH ،( و قد أظهرت تفاعلات موجبة شديدة و موجبة في الأعـضاء الفعالـة وظيفياً كالعضلات الطولية و الطبقة السطحية للبشرة و النسيج الميزانشيمي و الغدد المحيـة فـضلاً عـن الأعضاء التكاثرية الأنثوية و الذكرية. و يستنتج من الدراسة الحالية أن وجود المواد الكربوهيدراتية في الأنسجة المختلفة للدودة الشريطية موضوع الدراسة تسهم في كل من الحركة و الامتصاص و تخزين الطاقة لديمومة تطفلها في مضيفها.
This research included the histochemical study of cestoda Bothriocephalus acheilognathi in order to clarifying the place of the carbohydrates in different tissues and organs of this cestoda, this can be done by the use of periodic acid shiffs (PAS), Tuluedene blue, alcian blue at PH 1.0, 2.5 techneque. It was noticed that the result is strong reactions in functional active organs in the longitudinal muscles, epidermis surface, mesenchyme tissue, vittelaria glands and male and female reproductive organs. This study concludes that the presence of carbohydrate substances in various tissues of the cestoda are to contribute to the movement, absorption and storage of energy for the parasitic in there host.
References used
Berrada-Rkhami, O.; Leducq, J. and Gabrion, C. (1990). Selective distribution of sugars on the tugmental surface of adult Bothriocephalus gregarious (Cestoda: Pseudophyllidea). Int. j. Parasitol.20:285-297
Brockerhoff, A. and Jones, M. (1995). Ultrastructure of the scolex and tentacles of the metacestode of polyprocephalus species (Cestoda: Lecanicephalidae) from the blue–swimmer crab Portunus pelagic. Int. J. Parasitol.25, 1077-1088
Burenina, E. A. (2007). Comparative and outogenic biochemistry. Zhurnal Evolyutsiomoi Biokhimili fiziologii , 43 (3) : 240-245
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و القاء الضوء على أهمية و دور التلوينات المناعية النسيجية الكيميائية في وضع تشخيص نهائي و ذلك وفقا لتصنيف عام 2008.
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