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Register a new userPhylogenetic relationships among Triticum L. and Aegilops L. species based on the internal transcribed spacer sequences of nrDNA (ITS)
العلاقات التطورية بين أنواع من Aegilops L. و Triticum L.باستخدام internal transcribed spacer sequences(ITS) nrDNA
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يهدف هذا البحث الى دراسة علاقات القرابة الوراثية بين أنواع من Aegilops L. و Triticum L. باستخدام internal transcribed spacer (ITS) و قد استخدم في هذه الدراسة 8 أنواع من Aegilops L. و 7 أنواع من Triticum L. تم تحليل التسلسل لمنطقة ((ITS باستخدام برنامج (CLUSTAL W 2.1 multiple sequence aligment program) و درست علاقات القرابة الوراثية بين الأنواع باستخدام المتوسط الحسابي للمجموعات الزوجية غير المزانة Unweighted Pair Group Mean Arithmetic Average (UPGMA) و. (NJ)and neighbor-joining.
The research Studies genetic relationships among Triticum L. and Aegilops L. species by direct sequencing of PCR-amplified internal transcribed spacer (ITS) of nuclear ribosomal DNA to investigate the polymorphism in nucleotide sequences among 8 Aegilops L. and 7 Triticum L. species. ITS sequences were aligned with CLUSTAL W 2.1 multiple sequence aligment program. The phylogenetic relationships among species were reconstructed using Unweighted Pair Group Mean Arithmetic Average (UPGMA) and neighbor-joining (NJ) methods.
References used
AL-AHMAR, E., HAIDER, N., AZZAM. H. 2010. Genetic Relationships among Aegilops L. Species Using DNA Molecular Markers. General Commission for Scientific Agricultural Research, Gene Bank Division, Faculty of Agriculture, Damascus University. Syria
BADAEVA, E., FRIEBE, B., GILL. B. 1996. Genome differentiation in Aegilops. Physical mapping of 5S and 18S–26S ribosomal RNA gene families in diploid species. Genome 39:1150–1158
F., CAMPELL, C. S., DONOGHUE, M. J., 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Mol. Bot. Gard.,82, 247–277
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