In this study are evaluated the effects of addition of vitamin E and Selenium on the sperm
motility and vitality of Syrian rooster, at storing at 4 C⁰ for 24 and 48 hours. The Results
showed that the addition of both vitamin E and E with Selenium i
mproved the sperm
vitality and movement, but the vibration movement was decreased during 24 and 48 hours.
Our results also showed that the concentration of (5mg E+0.6mg Se) gave the highest
value of sperm vitality, rapid and slow movement with 17.79%, 10.65%, 8. 28%
respectively for 24 hours, and 19.29%, 10.06% 10.37% respectively for 48 hours. Whereas
the concentration of (10 mgE+0.6 Se) gave the lowest values of mentionned parameters.
Contrarily, the first concentration gave the lowest and the second the highest values of the
vibration movement. Finally, the statistical analysis (p<0.001) showed a significant
difference between studied parameters.
This study was carried out at the laboratory of artificial insemination and
embryo transfer in Ezra station Belongs to ACSAD during 2009 to estimate the
efficiency of local diluents (sodium citrate with egg yolk, EYC and skimmed
milk, SM)in compar
ison to Andromed® as a standard diluent for short and long
-term preservation period of semen in Awassi rams. Four rams, two years old
with live body weight 75± 3 Kg, were used. Semen was collected by artificial
insemination twice/ per day per week for 4 months during the breeding season.
Progressive motility and live – dead sperms were evaluated at each step of
semen handling. Results showed that there was a significant effect (p<0.05) for
the ram on ejaculate volume (1.8-2.46 ml) and sperm concentration (2.4-3.8 X
109/ml) and more significant effect (p< 0.01) for the diluents on studied traits
after each step of semen handling. The highest final survival rate of viability
(65.1%) was shown in semen diluted with Andromed® compared with 51.3% in
EYC and 22.3% in SM and the total rates of dead sperms were 41.9, 56.9.
78.9% in the standard, EYC and in SM, respectively. It was concluded that
local diluents can be used for extending and using Awassi semen as a fresh
diluted semen for a short time preservation using SM and as a frozen and
preserved semen for a long time with EYC in artificial insemination programs
and it was suggested that EYC with glycerol can be replaced for Andromed® if
sperm concentrate is increased by 10-15% at dilution.
Determination of infertility cause and the quality of sperms in semen are the
most important factors when we want to choose the suitable method of therapy
of assisted reproduction techniques, which has been proven to be the most
effective method t
oday in the treatment of many cases of male infertility, and it
allows to choose the best sperm for Intracytoplasmic sperm injection technique
(ICSI).
In our study we tried to find a relationship between one of the semen
parameters with the other basic parameters, we studied the concentration
motility and morphology of sperms of 23 infertile men with oligozoospermia,
and 5 control cases for fertile men.
This Study was carried-out at Shami Goat Improvement Station in Karahta.
Ten growing Shami male goats, aged 8 months, housed and managed under the
same conditions were used to study the semen characteristics. Artificial vagina
was used to collect
semen at weekly intervals, for 13 months to determine the
volume, motility, pH, and sperm concentration using Neubaure- Spectrophotometer
and Spermiodensimeter techniques.
Overall averages and standard errors of volume, motility, concentration,
and pH were 0.9 ± 0.07 ml, 65.24 ± 1.41%, 3.57 ± 0.14 billion/ml, and 6.52 ±
0.07, respectively. Seasonal variations were observed. A positive significant
correlation between volume and motility (r= 0.42, P<0.01) was found, while
negative correlations (P<0.01) between pH and other studied semen
characteristics were recorded.
Linear equations for detecting sperm concentration as measured by either,
Spermiodensimeter or Spectrophotometer were obtained.