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In this study are evaluated the effects of addition of vitamin E and Selenium on the sperm motility and vitality of Syrian rooster, at storing at 4 C⁰ for 24 and 48 hours. The Results showed that the addition of both vitamin E and E with Selenium i mproved the sperm vitality and movement, but the vibration movement was decreased during 24 and 48 hours. Our results also showed that the concentration of (5mg E+0.6mg Se) gave the highest value of sperm vitality, rapid and slow movement with 17.79%, 10.65%, 8. 28% respectively for 24 hours, and 19.29%, 10.06% 10.37% respectively for 48 hours. Whereas the concentration of (10 mgE+0.6 Se) gave the lowest values of mentionned parameters. Contrarily, the first concentration gave the lowest and the second the highest values of the vibration movement. Finally, the statistical analysis (p<0.001) showed a significant difference between studied parameters.
This study was carried out at the laboratory of artificial insemination and embryo transfer in Ezra station Belongs to ACSAD during 2009 to estimate the efficiency of local diluents (sodium citrate with egg yolk, EYC and skimmed milk, SM)in compar ison to Andromed® as a standard diluent for short and long -term preservation period of semen in Awassi rams. Four rams, two years old with live body weight 75± 3 Kg, were used. Semen was collected by artificial insemination twice/ per day per week for 4 months during the breeding season. Progressive motility and live – dead sperms were evaluated at each step of semen handling. Results showed that there was a significant effect (p<0.05) for the ram on ejaculate volume (1.8-2.46 ml) and sperm concentration (2.4-3.8 X 109/ml) and more significant effect (p< 0.01) for the diluents on studied traits after each step of semen handling. The highest final survival rate of viability (65.1%) was shown in semen diluted with Andromed® compared with 51.3% in EYC and 22.3% in SM and the total rates of dead sperms were 41.9, 56.9. 78.9% in the standard, EYC and in SM, respectively. It was concluded that local diluents can be used for extending and using Awassi semen as a fresh diluted semen for a short time preservation using SM and as a frozen and preserved semen for a long time with EYC in artificial insemination programs and it was suggested that EYC with glycerol can be replaced for Andromed® if sperm concentrate is increased by 10-15% at dilution.
Determination of infertility cause and the quality of sperms in semen are the most important factors when we want to choose the suitable method of therapy of assisted reproduction techniques, which has been proven to be the most effective method t oday in the treatment of many cases of male infertility, and it allows to choose the best sperm for Intracytoplasmic sperm injection technique (ICSI). In our study we tried to find a relationship between one of the semen parameters with the other basic parameters, we studied the concentration motility and morphology of sperms of 23 infertile men with oligozoospermia, and 5 control cases for fertile men.
This Study was carried-out at Shami Goat Improvement Station in Karahta. Ten growing Shami male goats, aged 8 months, housed and managed under the same conditions were used to study the semen characteristics. Artificial vagina was used to collect semen at weekly intervals, for 13 months to determine the volume, motility, pH, and sperm concentration using Neubaure- Spectrophotometer and Spermiodensimeter techniques. Overall averages and standard errors of volume, motility, concentration, and pH were 0.9 ± 0.07 ml, 65.24 ± 1.41%, 3.57 ± 0.14 billion/ml, and 6.52 ± 0.07, respectively. Seasonal variations were observed. A positive significant correlation between volume and motility (r= 0.42, P<0.01) was found, while negative correlations (P<0.01) between pH and other studied semen characteristics were recorded. Linear equations for detecting sperm concentration as measured by either, Spermiodensimeter or Spectrophotometer were obtained.
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