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Register a new userEfficiency of some diluents in cryopreservation of Awassi Ram Semen
فعالية بعض محاليل التمديد في حفظ السائل المنوي لكباش العواس
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نفذت الدراسة في مخبر التلقيح الصنعي و نقل الأجنة في محطة بحوث إزرع التابعة للمركز العربـي لدراسات المناطق الجافة و الأراضي القاحلة (أكساد)، خلال العام 2009 ، بهدف مقارنـة فعاليـة محاليـل R التمديد المحلية (سترات الصوديوم مع صفار البيض، و الحليب الخالي الدسم)، مـع الــ Andromed كمحلول قياسي مستورد لحفظ السائل المنوي لكباش العواس لفترات قصيرة أو طويلة الأمد. جمع الـسائل المنوي من أربعة ذكور بعمر سنتين، و بمتوسط وزن 75 ±3 كغ باستخدام المهبل الصنعي مرة أسبوعيا، بمعدل قذفتين/ مرة، مدة 4 أشهر خلال الفصل التناسلي. قيمـت الحركـة التقدميـة للنطـف مجهريـاً، و استعملت صبغات خاصة لتمييز النطف الميتة عند كل مرحلة من مراحل مداولة السائل المنوي. أظهـرت النتائج وجود تأثير معنوي (P> 05.0) للكبش في حجم القذفـة (8.1–46.2 مـل)، و تركيـز النطـف (4.2 -8.3 مليار نطفة/مل)، كما أثّر محلول التمديد بدرجة كبيرة (p> 01.0) فـي الحركـة التقدميـة للنطف و نسبتها الميتة خلال مراحل مداولة السائل المنوي، و كان معدل البقاء الكلي للنطـف الحيـة فـي محلول الشاهد (1.65%) و أقلها في محلول تمديد الحليب (3.22%) أما في محلول الـسترات فبلغـت 3.51 % و بلغت النسبة الكلية للنطف الميتة 9.41 ، و 9.56 ، و 9.78 % للشاهد، و السترات، و الحليـب، على التوالي. و استُنتِج أنه يمكن اعتماد محاليل التمديد المصنعة محلياً في تمديد السائل المنـوي لكبـاش العواس بشكل طازج و ممدد، و حفظه لفترات قصيرة (محلول الحليب الخـالي الدسـم)، و بـشكل مجمـد، و حفظه لفترات طويلة (السترات مع صفار البيض). و يوصي باستخدام محلول السترات مع صفار البـيض و الغليسيرول بديلاً عن محلول الأندرومد المستورد خاصة إذا زيد تركيز النطف قبل التجميـد بنحـو 10- 15 % في برامج التلقيح الصنعي في الغنم.
This study was carried out at the laboratory of artificial insemination and embryo transfer in Ezra station Belongs to ACSAD during 2009 to estimate the efficiency of local diluents (sodium citrate with egg yolk, EYC and skimmed milk, SM)in comparison to Andromed® as a standard diluent for short and long -term preservation period of semen in Awassi rams. Four rams, two years old with live body weight 75± 3 Kg, were used. Semen was collected by artificial insemination twice/ per day per week for 4 months during the breeding season. Progressive motility and live – dead sperms were evaluated at each step of semen handling. Results showed that there was a significant effect (p<0.05) for the ram on ejaculate volume (1.8-2.46 ml) and sperm concentration (2.4-3.8 X 109/ml) and more significant effect (p< 0.01) for the diluents on studied traits after each step of semen handling. The highest final survival rate of viability (65.1%) was shown in semen diluted with Andromed® compared with 51.3% in EYC and 22.3% in SM and the total rates of dead sperms were 41.9, 56.9. 78.9% in the standard, EYC and in SM, respectively. It was concluded that local diluents can be used for extending and using Awassi semen as a fresh diluted semen for a short time preservation using SM and as a frozen and preserved semen for a long time with EYC in artificial insemination programs and it was suggested that EYC with glycerol can be replaced for Andromed® if sperm concentrate is increased by 10-15% at dilution.
References used
Amdal, J., K. E. Johansen and T. Grafter. 1982. Artificial insemination of ewes using frozen semen. Proc. 14th Nord. Vet. Cong., Copenhagen; 349-350
Abdel- Hakeam, A. A., E. F. Graham, I. A. Vazquez and K. M. Chaloner. 1991. Studies on the absence of glycerol in unfrozen and frozen ram semen. Development of an extender for freezing: Effects of osmotic pressure, egg yolk levels, type of sugars, and the method of dilution. Cryobiology; 28: 43- 49
Al- Juburi, S. A. 1987. Studies on preservation of ram semen by freezing and other methods. Ph. D. Faculty of agric. Zagazig univ
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This Study was carried-out at Shami Goat Improvement Station in Karahta.
Ten growing Shami male goats, aged 8 months, housed and managed under the
same conditions were used to study the semen characteristics. Artificial vagina
was used to collect
semen at weekly intervals, for 13 months to determine the
volume, motility, pH, and sperm concentration using Neubaure- Spectrophotometer
and Spermiodensimeter techniques.
Overall averages and standard errors of volume, motility, concentration,
and pH were 0.9 ± 0.07 ml, 65.24 ± 1.41%, 3.57 ± 0.14 billion/ml, and 6.52 ±
0.07, respectively. Seasonal variations were observed. A positive significant
correlation between volume and motility (r= 0.42, P<0.01) was found, while
negative correlations (P<0.01) between pH and other studied semen
characteristics were recorded.
Linear equations for detecting sperm concentration as measured by either,
Spermiodensimeter or Spectrophotometer were obtained.
١٢٠ -Awassi ewes, ٣٦ ± ٢,٠ mos. old, ٤٢,٥ ± ٢,٥ kg. weight were used to
know the efficiency of artificial insemination (AI) in reproductive performance
of hormonal treated Awassi ewes in breeding season.
Thirty weaned Awassi lambs (٧٥ days old), reared in the metabolic unit at
the College of Agriculture, University of Damascus, were randomly divided into
٥ groups to study the effect of consumed crude protein level available in the
rations on the daily gain rate, live body weight and the food converting rate.
This research was done as a collaboration work between Tishreen University and the
General Commission for Scientific Agricultural Research in Karahta research station for
improvement of Shami goats and Der Al-Hajar station to set tables for normal
values of
some blood and chemical parameters in Awassi sheep during pregnancy to predict any
nutrition or health disorders. Forty seven Awassi ewes in the same age (in the third season)
were used for that purpose. Blood sample were collected from all ewes every month of the
pregnancy period. Thealanine aminotransferase (ALT),the aspartate aminotransferase
(AST), the alkaline phosphatase (ALP), the glucose (Glu) were analyzed.
Results indicated the existence of significant changes (P<0.05) between the
increase and decrease in all indicators studied throughout The study period, which shows
the change in functional status of liver in conjunction with stage of pregnancy.The overall
average of the effectiveness of enzymes (2.05-26.47), (32.65-181.40) and (11.17-79.76)
U/l for each of the ALP, ALT and AST, respectively, and total concentration of glucose
(37,50-95.20 mg/dl) throughout the study period.
The aim of this investigation is to preserve fresh Awassi lamb meat at
different storage conditions.
The Awassi lamb from the thigh part of fresh meat was stored in
Vacuuming Polyethylene Packaged with 100% CO2, 100% N2 and
100% vacuuming and at
temperature +3 ºC and +7 ºC.
The study showed that the meat can be stored at temperature +3ºC between
20 -30 days for the CO2 and vacuuming, and between 10-20 days for the N2 .
The study also indicated that the meat can be stored at temperature +7 ºC
between 10-20 days for CO2, and for 10 days under N2, and up to 10 days for
the vacuuming without altering the organoleptic properties.
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