In this study we isolated chlorella vulgaris alage from some water
areas , and test effect some conditions (light intensity,Nitrat and
phosphate concentrations ,solution soil) on its Growth Range and
Duplicated time the cells.
The effect of different concentrations of nitrate and phosphate and soil extract on the
total growth and chemical composition to the green algae Chorella vulgaris isolated from
some localaquatic habitats had studied in this research, at three type
of temperature (30 ,
25, 20), the maximum total growth reached up to 4.66 Cell/h and the lowest duplicated
time was 1.55 h at temperature 25ºC and at concentration of nitrate 1 g/L , 0.05 g/L of
phosphate, and 10/100 mL of soil solution , while the number of cells reached to 59423,61
cell/mL at the day 22 of cultivation.
The chemical composition differed by the changing of temperature and nutrient
medium composition , while the maximum protein content reached to 48.36 % at the
maximum concentration of nutrients and 25 ºC of temperature , the maximal concentration
of lipid was 19,78 % at temperature 25ºC and concentration of nitrate 0.1 g/L and 0.01 of
phosphate and 6/100 mL of soil solution , the concentration of carbohydrates were the
lowest where the maximal ratio was 17.41 % at temperature 20ºC .
According to the algae ability in photosynthesis and carbohydrate production with
high value in nutrition and industry, this research was carried out during 2015 to study the
qualitative and quantitative content of carbohydrates in Chlorella vulgar
is which was
isolated from southern Syrian fresh water, and to determine the effect of the growth
conditions; temperature (15, 25, 35 C), sodium nitrate concentration (0.1, 0.25, 0.4 g/l) and
pH (6, 7, 8) on that content. The results showed that the total content was in the range of
20% to 40%;with galactose and rhamnose dominating among the rest of sugars. The
temperature 15°C was the best to get the greatest total content with the highest value of
starch, cellulose, hemicellulose and pectin. In addition, the low nitrate concentration 0.1 g/l
was combined with the highest content of pectin and its compounds, while the glucose
content was not affected by the nitrogen concentration. Related to pH effect, the acidic
media was proved to be the best for the highest carbohydrate content. Moreover, cellulose,
starch, and pectin increased in the alkali medium over the rest of carbohydrates.
