A successful and detailed in vitro propagation system for rapid
micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has
been developed. Shoot tips and axillary buds excised from field-grown trees
were used as explants, which were surface-disinfected with mercury bichloride,
or with solution of sodium or calcium hypochlorite followed by three rinses
with sterile distilled water.
The study was performed over the years (2014٬2015) in Barshin
Research Station- General Commission for Scientific Agriculture
Research on the trees age of 15 in grafted with Golden Delicious
Cultivar on two rootstocks, strong: seeds ( Malus domest
ica) and half
strong (vegetative Malling-Merton "MM111"). The study focused on the
effect of the original on the concentration of each of the elements (N-P)
in the leaves and branches of the Golden delicious Cultivar grafted on
those rootstocks.