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A successful and detailed in vitro propagation system for rapid micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has been developed. Shoot tips and axillary buds excised from field-grown trees were used as explants, which were surface-disinfected with mercury bichloride, or with solution of sodium or calcium hypochlorite followed by three rinses with sterile distilled water.
The study was performed over the years (2014٬2015) in Barshin Research Station- General Commission for Scientific Agriculture Research on the trees age of 15 in grafted with Golden Delicious Cultivar on two rootstocks, strong: seeds ( Malus domest ica) and half strong (vegetative Malling-Merton "MM111"). The study focused on the effect of the original on the concentration of each of the elements (N-P) in the leaves and branches of the Golden delicious Cultivar grafted on those rootstocks.
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