Several strains of leishmania were isolated from patients suffering
from cutaneous leishmaniasis in Damascus countryside; and cultured in
vitro on semisolid medium (agar with rabbits blood) at first, then in
monophasic RPMI-١٦٤٠ medium supplemente
d with ١٠٪ (v/v) heatinactivated
fetal calf serum to determine the ideal conditions of culture.
The types of these strains were specified as Leishmania major using
isoenzymatic electrophoresis.
