Spices are becoming increasingly important world-wide due to their effectiveness as natural antibiotics, the thing that makes the world nowadays tend to use spices instead of chemical drugs. However, this doesn’t mean they are free of some dangerous
pathogens such as Klebsiella spp. which can lead to a wide range of illnesses, notably pneumonia, urinary tract infections (UTIs), and bacteremia. Although we don’t know the natural habitat of this bacterium we find that it thrives in herbs and spices, concluding thus that plants may be its natural habitat.
The primary isolation of this bacterium was based first on its growth on general media and then isolation on selective media. Colonies of the microorganisms were counted on different media then Klebsiella was isolated from a selective media. The isolated colonies of Klebsiella were circular, dome-shaped, 3-4 mm in diameter, with mucoid sticky edges, and surrounded by capsule. After this we chose the typical colony and identified it using Polymerase chain reaction PCR and biochemical tests. Our results showed that 32% of examined specimen contained Klebsiella spp.
Leishmaniasis spreads in eighty-eight countries, including the Syrian Arab
Republic. This disease is caused by a protozoic parasite of the genus
Leishmania. Twenty-two species of leishmania were reported to be pathogenic
for human. The disease is
presented in three clinical forms: cutaneous,
mucocutaneous and visceral leishmaniasis. A set of methods for diagnosis and
identifying the type of parasite have been developed in order to give
appropriate treatment. These methods include isoenzyme analysis, serological
and immunological methods and DNA hybridization. The polymerase chain
reaction (PCR), using genomic or kinetoplastid DNA; provides an excellent tool
for diagnosis and characterization of leishmania species. In this work, the two
types of DNA were extracted in one step from cultured isolates taken from
patients. We have used kinetoplastid DNA and specific primers to determine
parasite species by PCR. These primers amplify species specific fragment. In
this study, we have determined that the causative species of the cutaneous
leishmaniasis in all patients was L.tropica.
Enterobacter sakazakii is considered an opportunistic pathogen that has
been associated with severe lethal infections especially in neonates, elderly, and
Immunocompromised adults. E. sakazakii is a Gram negative, facultative
anaerobes rod-shaped
bacterium. It belongs to the family Enterobacteriaceae
and genus Enterobacter.
Although we don’t know the natural habitat of this bacteria we find that it
exists in high rate in herbs and spices which indicates that plant may be this
natural habitat.
Brucellosis is an endemic zoonosis in Syria, affecting large numbers of
animals and an increasing number of humans. Brucella is an intracellular
pathogen capable of infecting animals and humans. The aim of this study was
to develop a polymerase ch
ain reaction (PCR) protocol for the detection of
Brucella melitensis in artificially contaminated cheese.
