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Register a new userCharacterization of Lipoxygenase which extraction and Purification From Peanut Seed
توصيف أنزيم الليبوكسيجينيز Lipoxygenase المستخلص و المنقى من بذور الفول السوداني
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وصف أنزيم الليبوكسيجينيز المستخلص من الفول السوداني بعد تنقيتـه فـي الدراسـة الـسابقة، و أشارت النتائج إلى أن الوزن الجزيئي للأنزيم هو 97 كيلو دالتن مقدراً بطريقة الترشيح الهلامي، و بلغت نقطة التعادل الكهربائي (PI) للأنزيم 2.6 ،كما أظهرت الدراسة أن الأنزيم من البروتينات الـسكرية و أن نسبة الجزء الكربوهيدراتي تشكل 13 % عند تقديرها بطريقة الفينول – حمض الكبريتيك.
The enzyme was characterized by the following: Its molecular weight was 97 KD as estimated by gel filtration, iso electric point was at 6.2. The result showed that it was a glycoprotein with a carbohydrate content of 13 % as determined by phenol – sulfuric acid method.
References used
Allen, J. S.; Angelo, J. G and Robert L. O. (1997). Enzymes and oxidative stability of peanut products, southern regional research Centers, U. S. Department of Agric., New Orleans , Accymopsium series 97
AL-Obaidy, H. M. (1975). Broad bean lipoxygenase, M. Sc. Thesis, Food Sci. Dept. Agricultural college, Baghdad University
AL-Obaidy, H. M and Siddiqi A. M. (1981). Properties of Braod bean Lipoxygenase, Journal of Food Science. V. (46): 622-629
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The study included the selection of the best methods to extract the enzyme
among nine methods. The protein content was concentrated and precipitated
by cold acetone among other five methods of concentration (partial
purification). The purification
stages were achieved by using ion exchange
column chromatography (DEAE – Sephadex A 50 column). Followed by gel
filtration chromatography using sephacryl S-300. The active parts were
lyophlizated (free drying) to obtain Lipoxygenase with 43.18% yield and 8.16
folds of purification and specific activity of 1162.9 unit / mg.
The purity of enzyme was confirmed by poly acryl amide gel
electrophoresis under non denaturing conditions, with the appearance a single
band .
Some characteristics of β-galactosidase enzyme that was isolated from a new
born goat brain were studied. This study concluded that the enzyme is glucoenzyme
in which the carbohydrate part constitutes 22.1% in accordance with
phenol –sulfate acid
method.
The optimum pH for the enzyme activity is 5.5. The enzyme lost its activity
completely at pH8.5, and showed great stability at the range of pH 4-6.
The results indicated that the optimum temperature for the enzyme activity
is 55Co at the optimum pH. The stability temperature for the enzyme is
35-60Co.
The analytical results of 5%lactose solution hydrolyzed by the enzyme have
indicated that the hydrolysis rate is between 40% after 60 minutes, to 95%
after 270 minutes.
The research was carried out during 2015 and 2016 seasons at Arab El-Mulk village,
Banias district, in Tartous Governorate, to study the effect of planting peanut seeds
size (mixture, small, medium and large), and spraying plants with bread yeast
(Saccaromyces serevisiae) concentration of (4%) in three treatments (control, once
and twice), on biological yield (BY), Economic yield (EY) and some physiological
indicators; yield index (YI), Harvest index (HI), crop index (CI) and seed index
(SI). The experiment design was set as split plot in a randomized complete block
with four replications. The seeds size considered the main factor, while spraying
yeast allocated the sub plots. The results showed that seed size caused significant
increases in all of the studied indicators. The large seeds gave better values of (BY)
210 g, (HI) 30.56%, (CI) 44.12%, (SI) 74.85 g, except (EY) compared with mix,
small and med seeds. Also the results showed that there were no significant increase
between mixture and medium seed size in (YE) and (HI), but there was a significant
increase in (BY) and (SI), on the other hand the treatments; spray yeast for once and
twice had significant increases compared to the control, and the twice yeast spraying
gave better values (215.22 g ,63.98 g, 29.64%, 42.37 % and 73.45g ) of (BY , EY
,HI, CI and SI) respectively, compared to the control. The interaction between seeds
size and bread yeast spraying caused a significant increase which achieved 15.78 %
in (BY), 23.99 % in (EY), 9.74 in (HI), 13.68% in (CY) and 7.87g in (SI) compared
to the interaction between untreated treatment and small seeds size.
This research was conducted during 2014 season at Miaar Shaker
plains in Tartous region, to study the effect of applied drought stress for
24 days in 4 growth stages of peanut crop, i.e., 1. Brunching stage, 2.
Flower initialing stage, 3. Pod form
ation stage, 4. Seed formation stage,
beside of control (full irrigation by 12 days intervals). And effect of
these treatments on some growth attributes and yield to peanut
genotype (Sahel).
β-galactosidase enzyme was isolated from the new born goat brain by nine
methods, It was found that the sodium acetate 0.2 Mole/Liter +0.2Mole/Liter
NaCl PH5 method have given the highest specific activity of crude enzyme in
comparison with the ot
her methods. Also, this enzyme was purified by using
four methods, the second one (cold acetone) was the butter. As a result the
purification fold was about 135.46 times and the yield about 77.14% by using
Sephacryl S200 (second step). This enzyme is 187.437 KDa as a molecular
weight.
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