تعد تقانة الأضداد النانوية من طرائق البيولوجيا الجزيئية الواعدة، التي تجري من خلال الهندسة الوراثية لنوع خاص من الأضداد ذات السلاسل الثقيلة فقط التي تتميز بها عائلة الجمال Camelidea, بحيث تُمكن من الحصول على بروتينات صغيرة الحجم تُدعى بالأضداد النانوية التي تمتاز بكونها عالية الثباتية و الانحلالية و قادرة على الارتباط بمستضدها النوعي. تُنسل مورثات الأضداد النانوية خلال مراحل إنتاجها ضمن بلاسميدات أنظمة التعبير البروتيني مما يسمح بإنتاجها بصورة مستمرة و ثابتة لاستخدامها في أهداف بحثية و تطبيقية.
Nanobody technology is considered as a promising molecular biology
technique performed by means of the genetic engineering of special type of
antibodies, existing exclusively in Camelidea. It enables the obtaining of small
proteins, referred to as Nanobodies, which are characterized by high stability
and solubility, are able to link to their specific antigens. After production, the
Nanobody genes are cloned within plasmids of protein expression in bacteria,
allowing their stable and continuous production for research and applied
purposes.
References used
Abbady, A. Q., Al-Daoude, A., Al-Mariri, A., Zarkawi, M., and Muyldermans, S. (2012). Chaperonin GroEL a Brucella immunodominant antigen identified using Nanobody and MALDI-TOF-MS technologies. Vet. Immunol. Immunopathol. 146, 254-263
Abbady, A. Q., Al-Mariri, A., Zarkawi, M., Al-Assad, A., and Muyldermans, S. (2011). Evaluation of a nanobody phage display library constructed from a Brucellaimmunised camel. Vet. Immunol. Immunopathol. 142, 49-56
Abo Assali, L., Al-Mariri, A., Hamad, E., and Abbady, A.Q. (2012). Immunodetection of the recombinant GroEL by the Nanobody NbBruc02. World J. Microbiol. Biotechnol. 28, 2987-2995
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