The research was conducted in order to test the effect of each of the
filtrate T. viride and chitosan and sodium benzoate in filamentous
growth pathogenic fungi F. oxysporum and F. solani, and their
impact in protecting the seeds of peppers from t
hese two fungi, and
then assess the effectiveness of peroxidase enzyme in pepper plants
from seeds treated with the above-mentioned compounds.
The antagonistic activity of liquid culture filtrate of Trichodermaharzianum at
different concentrations (5, 10, 20 and 30%) was evaluated "in vitro" at 25 C° against
following plant and human pathogenic fungi: Aspergi
llusniger, Rhizopusstolonifer,
Fusariumoxysporum, F.moniliforme, Alternariaalternata and Candida albicans.
The results showed high level activity of culture filtrate inhibiting the growth of
tested fungi.
The activity was varied with the different species of fungi and the different
concentrations of culture filtrate. The highest activity was against fungus A.niger which
showed precent inhibition equal 96.3% at 30% concentration. Whereas the lowest
inhibition percentage to 77.6% was recorded for fungus A.alternata in comparison with
other fungi.
The culture filterate was not affected the radial colonies growth of R.stolonifer at 5%
and 10% concentrations, however the colonies was fragile and weak, and clearly inhibited
the spores formation.
The culture filtrate was showed high antagonistic capability against the human
pathogenic fungus C.albicans, and the radial inhibition growth was 1.38 cm at 30%
concentration.
