A simple and low cost thin layer chromatographic (TLC)-image analysis method was developed for rapid determination and quantification of monosodium glutamate (MSG) in some food samples. Chromatographic separation of MSG was achieved on silica gel TLC
plates, using n-butanol:glacial acetic acid:water (5:3:1, v/v/v) as the mobile phase and ninhydrin for spot detection. Image analysis of the scanned TLC plate was performed to quantify the amount of MSG, the method was validated and found to be accurate specific reliable and convenient for the analysis of MSG in some food sample.
The objective of this research to study the chemical composition and microbial load
for some types of pies by performing various chemical and microbial analysis of samples
collected, from different parts of the Syrian coast which are famous for its
production.
This study showed a large variation in the chemical composition of samples by type
of the pastry and place brought from, for example, the proportion of dry matter for peppers
and chard pies collected from Banias was, 75.71% and 44.90%, respectively, and the
percentage of dry matter was 73.88% and 76.18% and the proportion of protein was,
17.76% and 24 .88% for cheese pies collected from Tartous and Latakia 1, respectively.
This study indicated that all the pies contain a high percentage of fat [the lowest in chard
pies (17.64%) and the highest in cheese pies (38.12%)]. Furthermore a high percentage of
sodium was found (the highest in cheese pies 15.31 mg / 100 g and lowest in pepper pies
7.82 mg / 100 g).
The study also showed that these meals meet international standards in terms of
content of lead, cadmium, Nevertheless they are poor in fiber content except for chard pies
which were the most balanced in terms of components, making it the best type of pies in
nutritional aspect.
Finally, the results showed that the microbial load was too high, as the total census of
bacteria was higher than 105 and the total census of yeasts and fungi was higher than 102.
In addition, all pies are not conformed to the Syrian legislation. The study also showed
absence of E.coli and St. aureus of all samples.
