PCNA is synthesized in early G1 and S-phases of cell cycle. Tight linkage to cell
proliferation has led to the investigation of its role in the evaluation of tumors for
prognosis. The aim of this study is to use computerized image analysis to measu
re
PCNA and CD31 antibodies in a series of canine melanocytic tumors to assess density
of marked cells by these antibodies, and to correlate density of marked cells with
malignant degree of these tumors through comparative study between CD31, PCNA
and microscopic aspect. 12 dog melanic tumors were diagnosed during the period
2001–2010 in Pathology Department USAMV Cluj-Napoca, for PCNA expression
study, and 10 samples of them were treated with CD31 marker by immunohistochemical
for comparative study.
The study aims to determine the Seroprevalence of anti-H. pylori antibodies in patients with peptic complaints using indirect immunofluorescence assay (IFA), and compare serology with histological examination of biopsies from upper gastrointestinal e
ndoscopy to evaluate the sensitivity and specificity of this assay.
The Study sample included 120 participants divided into two groups: 69 patients with peptic complaints who underwent upper GI endoscopy in Al-Assad Hospital in Lattakia (average age 47 years old), and 51 volunteers without peptic complaints (average age 33 years old). The study was conducted in the period between February 2011 and March 2012. Indirect immunofluorescence assay (IFA) was used to study the seroprevalence of anti-H. pylori antibodies and fluorescence intensity in the two groups.
The results showed that 63 of the patients group (91.3%) and 39 of volunteers (76.47%) are anti-H. pylori antibodies positive. The comparison of serology with histological examination of biopsies showed a sensitivity and specificity of 78% and 80% respectively in the presence of moderate and strong degree of fluorescence. The results reveal the importance of IFA as a non-invasive screening test besides the endoscopy, and the utility of this technique for the epidemiologic studies in a local setting.
Patients infected with different species of Leishmania in Syria develop
specific antibodies. For this reason, we have developed an immunodot assay for
the serodiagnosis of cutaneous and visceral leishmaniasis, which utilizes antihuman
IgG conjugat
ed with peroxidase (HRP) as the visualizing agent.