Effect of media culture composition on In Vitro germination and growth of seeds of Heliotropium hirsutissimum Grauer

The effect of different culture medias (water, MS solid, MS 1/2 solid) on germination and growth of heliotropiun hirsutissimum Grauer. in vitro was studied with different concentrations of gibberellic acid (0.001-0.01-0.1 and 1 mg/l). The results showed that best germination rate (80.5%) and root (20mm) and shoot (35mm) growth were obtained in MS solid medium with 0.1mg/l GA3. Addition of 0.1mg/l GA3 to MS1/2 solid medium improved germination rate (75.44%), root (19mm), and shoot (24mm)growth. When GA3 (0.1 mg/l) was added to water medium, germination rate reached (65.33%), as well as root and shoot growth (12.66mm and20mm) alternatively after 2 weeks of planting. Seedling of MS solid medium were transferred into pots contained torp medium to adapting them with outside environment, and then surviving their growth until maturity after 4 weeks.

Effect of In vitro maturation, fertilization and culture media on bovine embryo production

This study was conducted at the biotechnology laboratory of AnimalWealth Research Administration, General Commission for Agricultural Research in order to compare the effect of different media on In vitro maturation (oocytes matured at 39 C° under 5% CO2 for 24 h), fertilization، and culture (7 days postfertilization) in humidified atmosphere 95% on bovine embryo production.