The aim of this in vitro study was to determine the effect of fissure
types on the microleakage, and penetration ability of fissure sealant.
The occlusal surface of ninety caries-free extracted human upper
premolars were cleaned, etched and sealed
with (UltraSeal XT
Plus) Sealant. The sealed teeth were thermocycled then coated with
two layers of nail varnish, leaving exposed a 1 mm window around
the sealant margins and then immersed in 1% methylene blue for 24
hours. Each tooth was sectioned bucco-lingually and examined with
a stereo microscope (40x magnification) for fissure types,
microleakage, and penetration ability.