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High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence length light sources are generally implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field of view (FOV). On the contrary, high temporal coherence light sources like lasers are capable of exploiting the full FOV of the QPM systems at the expense of less spatial phase sensitivity. In the present work, we employed pseudo-thermal light source (PTLS) in QPM which overcomes the limitations of conventional light sources. The capabilities of PTLS over conventional light sources are systematically studied and demonstrated on various test objects like USAF resolution chart and thin optical waveguide (height ~ 8 nm). The spatial phase sensitivity of QPM in case of PTLS is measured to be equivalent to that for white light source. The high-speed and large FOV capabilities of PTLS based QPM is demonstrated by high-speed imaging of live sperm cells that is limited by the camera speed and by imaging extra-ordinary large FOV phase imaging on histopathology placenta tissue samples.
Quantitative phase microscopy (QPM) has found significant applications in the field of biomedical imaging which works on the principle of interferometry. The theory behind achieving interference in QPM with conventional light sources such as white li
Mid-infrared photothermal (MIP) microscopy has been a promising label-free chemical imaging technique for functional characterization of specimens owing to its enhanced spatial resolution and high specificity. Recently developed wide-field MIP imagin
We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves. Using a Mirau interference objective and low-amplitude vertical sample vi
We propose and experimentally demonstrate a method of polarization-sensitive quantitative phase imaging using two photo detectors. Instead of recording wide-field interference patterns, finding the modulation patterns maximizing focused intensities i
Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a multitude of polarization light microscopy techniques are often used to access steady-state properties of birefringent sa