يستخدم Allelopathy ، كنباتات مستهدفة ، الأنواع المزروعة التي تعتبر في بعض الأحيان مفيدة للتحليل المقارن ، ولكن لا تتعايش بشكل طبيعي مع الأنواع المانحة (Reigosa et al. ، 2013). لذلك ، تم اختيار عشبة البقلة من الأعشاب الضارة (portulaca oleracea) (Portulacaceae) كهدف بالإضافة إلى الفصة(Medicago sativa) (Fabaceae) للمقارنة به. تم اختيار نوعين من الأعشاب الضارة عالمية التوزع وهي: الشوفان العقيم (Avena sterilis) ، وعشب حلب (الرزين) Sorghum halepense (Poaceae) كأنواع مانحة ، وتم إعداد مستخلصات المياه من النباتات المانحة واستخدام تراكيز 2 ٪ ، 4 ٪ ، 8 ٪ والمياه المقطرة للشاهد. تمت دراسة إنبات البذور ونمو البادرات المبكر للنباتات المستهدفة في أطباق بتري. تم قياس أطوال البادرات، بعد ذلك ، تم حساب المؤشرات التالية: إنبات البذور النسبي (RSG) ، نسبة الاستطالة النسبية للسويقة (RERs) والجذير (RERr) ، نسبة التثبيط للإنبات في طول السويقة وطول الجذير (I) ، ونسبة السويقة/ الجذير. تم حساب المتوسطات والانحراف المعياري وتم تحليل المتوسطات باستخدام ANOVA ، وتم اختيار اختبار LSD لمقارنة الاختلافات بين المعالجات عند مستويات الاحتمال (0.01 ، 0.05). أظهرت النتيجة أن المستخلصات كانت لها تأثيرات أكبر على نمو البادرات وليس على الإنبات وتأثرت جميع القياسات ، وكانت المستخلصات لها آثار تثبيط على طول الجذير للنوعين المختبرين ، وطول السويقة من الفصة، ولكن لها تأثير تحفيزي على طول السويقة في عشبة البقلة. كانت جميع نسب السويقة/ الجذير معنويةفي جميع التراكيز ، في حين كانت فروق إنبات البذور غير معنوية بشكل عام ، كانت لمستخلصات الرزين فعالية أكثر من مثيلتها في الشوفان العقيم ، وزاد التأثير بزيادة التركيز.
Allelopathy uses, as target plants, the cultivated species that are sometimes considered useful for comparative analysis, but do not naturally co-exist with the donor species (Reigosa et al., 2013) . Therefore, the weed common purslane (portulaca oleracea) (Portulacaceae) was selected as a target in addition to alfalfa (Medicago sativa) (Fabaceae) to compare with it. Two worldwide distribution weeds: sterile oat (Avena sterilis), and Aleppo grass (Sorghum halepense) (Poaceae) were chosen as donor plants, Water extracts of donor plants were prepared, 2%,4%,8% concentrations were used and distilled water was used for control. Seed germination and early seedling growth of target plants were studied in petri dishes. Lengths of seedlings were measured, after that, following indicators were calculated: Relative seed germination (RSG), relative elongation ratio of Shoot (RERs) and root (RERr), Inhibition percentage of germination, shoot and root length (I), and root/shoot ratio. Means and standard deviation were calculated and means were statically analyzed with one – way ANOVA, LSD test was chosen to compare differences between treatment means at a probability levels (0.01, 0.05). The result showed that extracts had greater effects on seedling growth rather than on germination and all measurements were affected, extracts had inhibition effects on root length of the two tested species, shoot length of alfalfa, but a stimulation effect on shoot length of common purslane. All root / shoot ratios were significant in all treatments of common purslane, and alfalfa treated with Aleppo grass only, while the seed germination differences were generally non – significant, Aleppo grass extracts were more effective than that of sterile oat, and the effect generally increased by increasing concentration.
References used
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