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Register a new userStudy Of Ability Of Proteus volgaris To Degrade Petroleum Alkane Chains Of Compounds Polluting Marine Environment
دراسة قدرة جراثيم المتقلبة الشائعة Proteus volgaris على تفكيك سلاسل الألكانات في المركبات النفطية الملوِّثة للبيئة البحرية
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تم عزل جراثيم عائدة لنوع المتقلبة الشائعة Proteus volgaris من المياه الشاطئية لمدينة اللاذقية (المرفأ) الملوثة بالمواد النفطية الناتجة عن نشاط النقل البحري، و اختبرت مقدرتها على تفكيك الألكانات النفطية الملوثة لهذه البيئة مخبرياً باستخدام تراكيز مختلفة من الملوثات النفطية. و استخدمت تقانة الكروماتوغرافيا الغازية (GC) لقياس تراكيز الألكانات النفطية. بينت النتائج أن الجراثيم المعزولة قادرة على تفكيك السلاسل الألكانية المختلفة، حيث كانت نسبة تفكيك كل من C12، C16، C18 هي 96.7%، 78.59%، 97.27 % على التوالي، بينما بلغت نسبة التفكك الاجمالي للسلاسل الألكانية 77.14% عند إضافة 10 ملغ/مل من الملوثات النفطية, بينما لم تتجاوز نسبة التفكك 17.26 % عند إضافة 75 ملغ/مل منها. و بالتالي فإن القدرة العالية لجراثيم Proteus volgaris على تفكيك السلاسل الألكانية الطويلة يؤهلها لتكون فاعلة في عملية المعالجة الحيوية Bioremediation للبيئة البحرية الملوثة.
Proteus volgaris was isolated from costal sea water of Lattakia city port, as it is contaminated with petroleum compositions coming from marine transportations activities. The ability of this bacteria to degrade the petroleum alkanes was studied by using different concentrations of petroleum as sole source of carbon and energy. The gas chromatography (GC) technique was used to measure the concentration of alkanes. Results showed that the isolated bacteria were able to degrade different alkanes chains. The ratio of biodegradation of C12, C16, C18 were 96.75%, 78.59%, 97.27% respectively, while the rate of biodegradation of the entire amount of alkanes was 77.14% in 10 mg/ml polluting concentration. The percentage of biodegradation at concentration 75 mg/ml of petroleum was 17.26%. Therefore, the high ability of Proteus volgaris to degrade the long chains alkanes recommended it to be effective in the bioremediation process of the contaminated marine environments.
References used
.BERNARD, D. JEREMIE, J.J. PASCALINE, H. First Assessment of Hydrocarbon Pollution In a Mangrove Estuary. Marine Pollution Bulletin, vol. 30,No 2, 146- 150, 1995
.CASTENHOLZ, R. W., Microbial mat research: the recent past and new perspectives, In: Stal L. J.,Caumette P. (Eds), Microbial mat, structure, development and environmental signification. NATO ASI series, Springer-Verlag, Brelin Heidelberg,. 3-18, 1994
.CEYHAN, N. Biodegradation of pyrene by a newly isolated Proteus vulgaris. Scientific Research and Essays Vol. 7(1), 66-77, 9 January, 2012
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The Palmyra's chain, is one of the Syrian strategic petroleum regions. Thus the
petroleum companies were interested by this structure through the petro-service contracts.
This study focused on petroleum potential of the Mizozoic formations in the N
Palmyra.
The formation thickness variations have permitted to estimate this potentiality
relating to: Kurachina dolomite, Butma, Al- Haramon, and Rutba formations. In the other
side, the other formations are poor. Also, through the results of executed studies, using the
time- map data and the seismic profiles analysis, a new promising structure, El Khashabia,
is proposed. In this structure a new well is suggested, which must be promising as
petroleum potential.
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ss and producing pieces for heating or producing barbecue charcoal. This products are with little smoke, smell and environment friendly. The results showed the following:
1- Producing pieces of byrene for heating with the possibility of controlling the strength and the press of pieces. With this we can contribule to environment protection and loosen the problem of heating in winter.
2- The comparison of organic fertilizer (Compost) from Byrene with mixture fertilizer from oak, straw and cock waste showed moral differences for nitrogen and kalium and no moral differences for organic substance, ashes, phosphourus and PH. The compost contains a little of Poly Phenolic. The SHETIANZY number for compost was with cold and hot water 0.277, 8.22, respectively and for mixture fertilizer 10, 16.
3- It was no moral differences between the specific heat and heat of combustion. The less moral difference was for both 0.430, 1.054, respectively. The barbecue charcoal from Dakka was with little smoke, smell and quick combustion. The producing cost of 1 kg was 5 syrian pounds
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galvanized in the laborators ( laboratorsies of the faculty of science) and in nature (on the
beach of the high-institute sea- research in Lattakia)
The Inula ext
racts were choosen as a plant inhibitor to cover the iron samples and
protect it . because this plant is well known in the Coastal area, also it,s very cheap and
easy to find and has good anti oxidation properties.
The best conditions for (Inula-seawater) extract were : (PH=7 , time =24 hours ,
concent ration = 10%).
The studied iron samples were treated with the ( inula-seawater) extract in the same
previous conditions and we studied the corrosion rate changes for those samples and the
affectivity of the inhibitor for them.
The results were compared in each stage with other samples that were not treated .
the treatment and after it were as follows:
The velocity of the corrosion in the iron samples (before the treatment with the
extract) in 120 days:
- in the laboratory tanks was 0.78 mg/cm2 .day, but after the treatment was 0.23
mg/cm2.day
-in the drizzle area was 0.80 mg/cm2.day, but after the treatment was 0.47
mg/cm2.day
-in the waves area was 0.75 mg/cm2.day , but after the treatment was 0.41
mg/cm2.day
- in the depth area was 0.79 mg/cm2.day , but after the treatment was 0.21
mg/cm2.day.
The following Fungal species were isolated from therhizosphere of wheat plant (TriticumaestivumL.): Trichodermaharzianum, T. viride, T. longibrachiatum, Rhizoctoniasolani, Alternariaalternata, Fusariumsolani, Fusariumoxysporum and Aspergillusniger. I
t was shown that all isolated fungi had the capacity to produce cellulase in solid medium (CMC- Agar), while the fungus T.viride gave the highest capability for the production of this enzyme(5.16). The fungus Rhizoctoniasolani gave less productive ratio (2.61). Quantitative test using Mandelium liquid medium showed that the fungus T.viride had the highest productivity of Cellulase(4.39 U/mL), and this fungus had greater biomass (8.96 g/L). The fungus Rhizoctoniasolani gave lower enzyme productivity(2.04 U/mL) and its biomass reached(4.65 g/L). The study of different carbon sources for the fungus T.harzianum showed that Sucrose was the best media in Cellulase productivity. It reached (3.87U/mL), and the biomass was (2.83 g/L).
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