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Study Of Ability Of Proteus volgaris To Degrade Petroleum Alkane Chains Of Compounds Polluting Marine Environment

دراسة قدرة جراثيم المتقلبة الشائعة Proteus volgaris على تفكيك سلاسل الألكانات في المركبات النفطية الملوِّثة للبيئة البحرية

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 Publication date 2013
and research's language is العربية
 Created by Shamra Editor




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Proteus volgaris was isolated from costal sea water of Lattakia city port, as it is contaminated with petroleum compositions coming from marine transportations activities. The ability of this bacteria to degrade the petroleum alkanes was studied by using different concentrations of petroleum as sole source of carbon and energy. The gas chromatography (GC) technique was used to measure the concentration of alkanes. Results showed that the isolated bacteria were able to degrade different alkanes chains. The ratio of biodegradation of C12, C16, C18 were 96.75%, 78.59%, 97.27% respectively, while the rate of biodegradation of the entire amount of alkanes was 77.14% in 10 mg/ml polluting concentration. The percentage of biodegradation at concentration 75 mg/ml of petroleum was 17.26%. Therefore, the high ability of Proteus volgaris to degrade the long chains alkanes recommended it to be effective in the bioremediation process of the contaminated marine environments.


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Research summary
تتناول الدراسة قدرة جراثيم المتقلبة الشائعة (Proteus volgaris) على تفكيك سلاسل الألكانات النفطية الملوثة للبيئة البحرية. تم عزل الجراثيم من مياه مرفأ مدينة اللاذقية الملوثة بالمواد النفطية الناتجة عن نشاط النقل البحري، واختبرت مقدرتها على تفكيك الألكانات النفطية باستخدام تراكيز مختلفة من الملوثات. استخدمت تقانة الكروماتوغرافيا الغازية (GC) لقياس تراكيز الألكانات النفطية. أظهرت النتائج أن الجراثيم المعزولة قادرة على تفكيك سلاسل الألكانات المختلفة بنسب متفاوتة، حيث بلغت نسبة التفكيك الإجمالية للسلاسل الألكانية 77.14% عند إضافة 10 ملغ/مل من الملوثات النفطية، بينما لم تتجاوز نسبة التفكيك 17.26% عند إضافة 75 ملغ/مل منها. تشير النتائج إلى أن الجراثيم قادرة على تفكيك السلاسل الألكانية الطويلة بكفاءة عالية، مما يؤهلها لتكون فعالة في عملية المعالجة الحيوية للبيئة البحرية الملوثة.
Critical review
دراسة نقدية: تعتبر هذه الدراسة خطوة مهمة في مجال المعالجة الحيوية للملوثات النفطية في البيئة البحرية. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، الدراسة تركز بشكل كبير على جرثومة واحدة فقط (Proteus volgaris)، بينما يمكن أن تكون هناك أنواع أخرى من الجراثيم التي قد تكون أكثر فعالية في تفكيك الألكانات النفطية. ثانياً، الدراسة تمت في ظروف مخبرية، وبالتالي قد تكون النتائج مختلفة في الظروف البيئية الحقيقية. أخيراً، لم تتناول الدراسة تأثير العوامل البيئية الأخرى مثل درجة الحرارة، الملوحة، والضغط على كفاءة التفكيك الحيوي.
Questions related to the research
  1. ما هي الجراثيم المستخدمة في الدراسة؟

    الجراثيم المستخدمة في الدراسة هي المتقلبة الشائعة (Proteus volgaris).

  2. ما هي التقنية المستخدمة لقياس تراكيز الألكانات النفطية؟

    تم استخدام تقانة الكروماتوغرافيا الغازية (GC) لقياس تراكيز الألكانات النفطية.

  3. ما هي نسبة التفكيك الإجمالية للسلاسل الألكانية عند إضافة 10 ملغ/مل من الملوثات النفطية؟

    بلغت نسبة التفكيك الإجمالية للسلاسل الألكانية 77.14% عند إضافة 10 ملغ/مل من الملوثات النفطية.

  4. ما هي التوصيات التي قدمتها الدراسة؟

    أوصت الدراسة بالتأكيد على أهمية التقنيات الحيوية في معالجة التلوث النفطي، والعمل على عزل أنواع جرثومية أخرى قادرة على عملية التفكيك، ومحاولة تطوير القدرة التفكيكية لهذه الأنواع باستخدام التقنيات الوراثية.


References used
.BERNARD, D. JEREMIE, J.J. PASCALINE, H. First Assessment of Hydrocarbon Pollution In a Mangrove Estuary. Marine Pollution Bulletin, vol. 30,No 2, 146- 150, 1995
.CASTENHOLZ, R. W., Microbial mat research: the recent past and new perspectives, In: Stal L. J.,Caumette P. (Eds), Microbial mat, structure, development and environmental signification. NATO ASI series, Springer-Verlag, Brelin Heidelberg,. 3-18, 1994
.CEYHAN, N. Biodegradation of pyrene by a newly isolated Proteus vulgaris. Scientific Research and Essays Vol. 7(1), 66-77, 9 January, 2012
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