Asthma exacerbations can be frequent and range in severity from relatively mild to
status asthmaticus. While the efficacy of intravenous MgSO4 hasbeen demonstrated, the
informations available on its inhaled role still low and conflicting, so the st
udy aim to
investigate the effect of thecombinationof MgSO4and salbutamol in the management of
acute asthma attack.
A randomized clinical trial was conducted on 76 patients, divided into two groups:
experimental (40) and control (36) patients. The trial received nebulised salbutamol with
magnesium sulphate and control nebulised salbutamol with saline serum 0.9% on three
sessions in 20 minutes. Signs of respiratory distress, wheezing, respiratory rate, pulse,
Sao2 and PEF were assessed.
Magnesium sulphate showed a significant effect in improving respiratory rate, Sao2
and PEF starting from the 20th minute( P <0.05), but did not observe the effect of MgSO4
in improving signs of respiratory distress, pulse and wheezing(P> 0,05), no side effects
attributed to magnesium sulphate were observed.Inhaled MgSO4, incombination with
salbutamol, appears to have benefits in the treatment of acute asthma attack.
Background & Objective: Spray drying techniques are used to produce inhaled powders of enzymes and proteins without denaturation. The surface free energy data of these powders is used to reduce the time of formulation development. During storage, the
surface free energy of the inhaled protein powders could change and so their performance will be affected. Many factors affect the limit of this change. Storage temperature is the critical factor.
Material& methods: A spray drying method was optimized to prepare inhaled spray dried lysozyme powders. Inverse gas chromatography was utilized to measure the surface free energy of the prepared powders pre and post-storage to detect the effect of storage temperature on the surface free energy.
Differential Scanning Calorimetry and biological activity tests were used to evaluate the integrity of lysozyme conformation after storage at different storage temperature.
Results: Increasing the storage temperature decreased both dispersive and specific surface components of the spray-dried lysozyme powders significantly (ANOVA: P < 0.001) up to 12% and up to 31 % after storage for one year at 40 °C, respectively. The overall decrease in the polar components resulted in a
reduction of the surface basicity. These decreases in the surface energetics would be due to the aggregation of denatured lysozyme molecules at the powder surface via hydrophobic regions rich with electrons. The same results were found after storage for one year at room temperature, but the extents of
changes were smaller.
Conclusion: It is advisable to store protein powders at 5°C to inhibit the changes in their surface free energy which is the crucial factor affecting their performance.
