The study was conducted to evaluate the efficiency of Pseudomonas chlororaphis MA342,
Serratia plymuthica HRO-C48K, Bacillus subtillis B2g, B. subtillis FZB27 to induce systemic
resistance in Tomato plants against Cucumber mosaic virus (CMV) under
greenhouse conditions.
Tomato seeds submerged in suspension of Pseudomonas chlororaphis MA342, Serratia
plymuthica HRO-C48, Bacillus subtillis B2g and B. subtillis FZB27 9×109cfu . plants emerged
from these seed were inoculated with CMV after 10 days of planting. The time of beginning
symptoms was recorded, Disease incidence, disease severity, area under disease progress curve
AUDPC, progress of infection disease degrees during the study period were calculated using
virtual symptoms . pyroxidas enzyme activity was determined, Some growth parameters such as
Plant height, foliage fresh weight, root fresh weight , foliage dry weight, root dry weight were
observed after 30 DAI. Results of this study showed that seed treatment with Pseudomonas
chlororaphis MA342, Serratia .plymuthica HRO-C48, Bacillus subtillis B2g, B. subtillis FZB27
significantly decreased the diseases incidence on treated and inculated plants which ranged
between 40% - 66.66% ,compared with un treated and inculated control 93.33% Without the
presence of significant differences between the bacterial treatments. significantly decreased the
diseases severity on treated plants which ranged between 45.53 %- 62.2% compared with inculated
and un treated control 88.86% .Without the presence of significant differences between the
bacterial treatments. Peroxidas activity on treated plants increased by Compared with untreated
plants. Seed treatments with bacteria significantly increased the plant growth. The plant height ,
fresh and dry weight of foliage, fresh and dry weight of root on treated plants were significantly
higher than those of inculated and un-treated plants according to LSD 5%.where bacteria reduced
the rate of stunting and the rate of decline mild and dry weight of foliage and mild and dry weight
of the root occurring due to infection with the Cucumber mosaic virus (CMV).
