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DNA was extraction from scanning microscopic slides to identification leishmaniases. Three methods used to DNA extraction, first one using ready extraction solvent (Promega kit), second depended on phenol-chloroform-iso propanol , whereas third one depended on columns with silica gel membranes (Qia gen kit). The results showed that (Promega kit) method didn’t gave high concentration of DNA. (Qia gen kit) method was the best for DNA concentration and purity
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