Thirteen viable and post - frozenthawed Shami cows embryos obtained by
super-ovulation and artificial insemination technique in order to determine sex
of embryos. Biopsies (1-2 cells) of embryos at morula or blastocyst stage were
taken using micro
manipulation under inverted microscope.
It was concluded that aspiration
of 1-2 blastomere (s) from embryos at morula or blastocyst stage was enough to
determine sex of embryo using PCR and it is recommended that using this
technique can be a useful tool in splitting and transferring of desired sex
embryos in genetic improvement program.
