Isolation And Cloning Of AapJ Gene From Brucella melitensis

Economic losses resulting from the impact of brucellosis on animals have increased, which is reflected in turn on humans. Brucella is pathogenic bacteria shared by humans and pet animals. They still pose a significant risk that threatens health and economics in Syria. Many efforts have focused to prevent the spread of brucellosis disease, by resorting to the use of vaccines prepared from Brucella strains, where now the target is preparation of vaccine components proteins of Brucella can be used as a vaccine or an improved traditional vaccine.

Construction of pRMES and pTMES plasmids to test their expression ability of Nanobodies via the efficient T7 promoter

Nanobody technology is considered as a promising molecular biology technique performed by means of the genetic engineering of special type of antibodies, existing exclusively in Camelidea. It enables the obtaining of small proteins, referred to as Nanobodies, which are characterized by high stability and solubility, are able to link to their specific antigens. After production, the Nanobody genes are cloned within plasmids of protein expression in bacteria, allowing their stable and continuous production for research and applied purposes.