The effect of different concentrations of nitrate and phosphate and soil extract on the
total growth and chemical composition to the green algae Chorella vulgaris isolated from
some localaquatic habitats had studied in this research, at three type
of temperature (30 ,
25, 20), the maximum total growth reached up to 4.66 Cell/h and the lowest duplicated
time was 1.55 h at temperature 25ºC and at concentration of nitrate 1 g/L , 0.05 g/L of
phosphate, and 10/100 mL of soil solution , while the number of cells reached to 59423,61
cell/mL at the day 22 of cultivation.
The chemical composition differed by the changing of temperature and nutrient
medium composition , while the maximum protein content reached to 48.36 % at the
maximum concentration of nutrients and 25 ºC of temperature , the maximal concentration
of lipid was 19,78 % at temperature 25ºC and concentration of nitrate 0.1 g/L and 0.01 of
phosphate and 6/100 mL of soil solution , the concentration of carbohydrates were the
lowest where the maximal ratio was 17.41 % at temperature 20ºC .
The effect of different concentrations of phosphate and nitrate and soil extracted on
the isolated growth isolation of Scendesmus acutus which is isolated from some local
aquatic areas has tested in this research, the number of cells and growth ran
ge and
duplicated time of all algal cultures among thirty five days has counted.
The highest growth range was 3.90 by using a mixture of the three solutions in
concentrations (1g/l nitrate + 0.05g/l phosphate + 10 ml / 100 of soil solution), the numbers
of cells reached to 8861.11 in the twenty fourth day of culture, and in the lowest duplicated
time 10.85, whereas the highest growth range was by using soil solution alone 2.86 and the
lowest duplicated time 2.13 at concentration 16/100ml, whereas the growth range
decreased and duplicated time increased by using nitrate and phosphate solution all by its
own, while the highest growth range reached to 2.13 and 1.76 and lowest duplicated time
3.39 and 4.11 in order.
In this study we isolated chlorella vulgaris alage from some water
areas , and test effect some conditions (light intensity,Nitrat and
phosphate concentrations ,solution soil) on its Growth Range and
Duplicated time the cells.
