This study were conducted on 450 samples of raw chicken meat
( 150 samples of thighs- 150 samples of breasts -150 samples of
wings ) were collected from retail market in Hama city, to detect
contamination of staphylococcus. Bacteriological and bio
chemical
tests results showed 407 samples positive for staphylococcus .
contained S. aureus 73 (16.2% ) isolated and coagulase-negative
Staphylococcus 334 (83.8%) isolated out of total samples. The
results of Multiplex PCR test conducted on S. aureus isolates,
showed that 37(50.7%) isolates harbored at least one enterotoxin
gene, with sea being the most frequently encountered ones.
This study has been conducted to adapt a quantitative RT-QPCR to
detect the least amount of virus and tissue culture pathogenic dose
(TCID50) of CAEV showed cytopathogenic effect (CPE) from
clinical samples.
The aim of this study is isolating brucella and using polymerase chain reaction technique for the molecular characterization of Brucellamelitensis,which causes caprine abortion. It also aimsto determine the rate of caprine abortion that is caused by
Brucellamelitensisin the Middle region of Syria. For this purpose 58 abortion samples were collected (36 aborted fetuses, 22 vaginal swabs). The results of bacterial isolation and polymerase chain reaction reveal that the prevalence of caprine abortion caused by Brucella melitensis was 53.4%. The study also reveals that using stomach contents from caprine aborted fetus can be the best site for brucella isolation. Running differentiation tests to distinguish field strains from vaccinal strains refer to the presence of 2 isolates(6.4%) belonging to vaccinal strains(Rev1) from 31 isolates of Brucella melitensis.
