The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
the leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania Tropica parasite , the hardness of
controlling the reservoirs of parasites, and there is no specific ,safe
active therapy. A Heat shock protein 20 HSP2
0 belongs to the
small HSP (sHSP) family.
Sublethal toxicity studies were conducted with a rotifera (Brachionus
calyciflorus) to determine the No observed effect concentrations (NOECs) of a
different eight toxic substances based on the protein mass reduction. Protein
mass (with moleculer
weight 43KDa - 67KDa) was quantified using
electrophoresis method. Quantitation of protein concentrations demonstrated
that toxicant stress reduced protein concentrations. The NOECs-43KDa and
NOECs - 67KDa were reported. Since in vivo protein cocentrations can be
assessed in a short time, these results can be obtained in only a few hours,
which is substantially less than traditional whole animal reproductive tests.
NOECs - NOECs - 43KDa test were generally more sensitive end points than
NOECs - NOECs-67KDa test and more sensitive than standard whole animal
toxicity tests for only four toxicants.
قمنا من خلال هذا البحث بتغطية بعض الجوانب المناعية للطفيلي، حيث عزل طفيلي الليشمانية من
أشخاص مصابين بداء الليشمانية الجلدي، من منطقة الرحيبة (ريف دمشق)، ثم استنبت الطفيلي في
البداية على وسط صلب N.N.N بدرجة حرارة ٢٦ ° مئوية، و من ثم على وسط سائل ١٦
٤٠ RPMI- بغية
تعيين الشروط المثلى للاستنبات (تركيز الطفيلي الأولي، و حجم الوسط)، فحصلنا على منحنى نمو نموذجي
لدى استخدام التركيز ٥×٥^١٠ طفيلي/ميلي لترًا، حيث يمر الطفيلي بأطوار نمو واضحة و منفصلة. لاحظنا
أيضًا التبدلات الشكلية التي تطرأ على الطفيلي أمامي السوط promastigote في كل طور.
