This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorgh
um, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
