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Deep penetration fluorescence imaging through dense yeast cells suspensions using Airy beams

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 نشر من قبل Yael Roichman
 تاريخ النشر 2019
  مجال البحث فيزياء
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We propose a new method to image fluorescent objects through turbid media base on Airy beam scanning. This is achieved by using the non-diffractive nature of Airy beams, namely their ability to maintain their shape while penetrating through a highly scattering medium. We show, that our technique can image fluorescent objects immersed in turbid media with higher resolution and signal to noise than confocal imaging. As proof-of-principle, we demonstrate imaging of 1$mu$m sized fluorescent beads through a dense suspension of yeast cells with an attenuation coefficient of 51cm$^{-1}$ at a depth of 90$mu$m. Finally, we demonstrate that our technique can also provide the depth of the imaged object without any additional sectioning.



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