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تسجيل مستخدم جديدMean velocity and effective diffusion constant for translocation of biopolymer chains across membrane
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Chaperone-assisted translocation through a nanopore embedded in membrane holds a prominent role in the transport of biopolymers. Inspired by classical Brownian ratchet, we develop a theoretical framework characterizing such translocation process through a master equation approach. In this framework, the polymer chain, provided with reversible binding of chaperones, undergoes forward/backward diffusion, which is rectified by chaperones. We drop the assumption of timescale separation and keep the length of a polymer chain finite, both of which happen to be the key points in most of the previous studies. Our framework makes it accessible to derive analytical expressions for mean translocation velocity and effective diffusion constant in stationary state, which is the basis of a comprehensive understanding towards the dynamics of such process. Generally, the translocation of polymer chain across membrane consists of three subprocesses: initiation, termination, and translocation of the main body part of a polymer chain, where the translocation of the main body part depends on the binding/unbinding kinetics of chaperones. That is the main concern of this study. Our results show that the increase of forward/backward diffusion rate of a polymer chain and the binding/unbinding ratio of chaperones both raise the mean translocation velocity of a polymer chain, and roughly speaking, the dependence of effective diffusion constant on these two factors achieves similar behavior.
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