ترغب بنشر مسار تعليمي؟ اضغط هنا

Stochastic modelling reveals mechanisms of metabolic heterogeneity

170   0   0.0 ( 0 )
 نشر من قبل Diego Oyarz\\'un
 تاريخ النشر 2019
  مجال البحث علم الأحياء
والبحث باللغة English




اسأل ChatGPT حول البحث

Phenotypic variation is a hallmark of cellular physiology. Metabolic heterogeneity, in particular, underpins single-cell phenomena such as microbial drug tolerance and growth variability. Much research has focussed on transcriptomic and proteomic heterogeneity, yet it remains unclear if such variation permeates to the metabolic state of a cell. Here we propose a stochastic model to show that complex forms of metabolic heterogeneity emerge from fluctuations in enzyme expression and catalysis. The analysis predicts clonal populations to split into two or more metabolically distinct subpopulations. We reveal mechanisms not seen in deterministic models, in which enzymes with unimodal expression distributions lead to metabolites with a bimodal or multimodal distribution across the population. Based on published data, the results suggest that metabolite heterogeneity may be more pervasive than previously thought. Our work casts light on links between gene expression and metabolism, and provides a theory to probe the sources of metabolite heterogeneity.



قيم البحث

اقرأ أيضاً

77 - Carl Graham 2020
We revisit the modeling of the diauxic growth of a pure microorganism on two distinct sugars which was first described by Monod. Most available models are deterministic and make the assumption that all cells of the microbial ecosystem behave homogene ously with respect to both sugars, all consuming the first one and then switching to the second when the first is exhausted. We propose here a stochastic model which describes what is called metabolic heterogeneity. It allows to consider small populations as in microfluidics as well as large populations where billions of individuals coexist in the medium in a batch or chemostat. We highlight the link between the stochastic model and the deterministic behavior in real large cultures using a large population approximation. Then the influence of model parameter values on model dynamics is studied, notably with respect to the lag-phase observed in real systems depending on the sugars on which the microorganism grows. It is shown that both metabolic parameters as well as initial conditions play a crucial role on system dynamics.
Metabolism plays a central role in cell physiology because it provides the molecular machinery for growth. At the genome-scale, metabolism is made up of thousands of reactions interacting with one another. Untangling this complexity is key to underst and how cells respond to genetic, environmental, or therapeutic perturbations. Here we discuss the roles of two complementary strategies for the analysis of genome-scale metabolic models: Flux Balance Analysis (FBA) and network science. While FBA estimates metabolic flux on the basis of an optimisation principle, network approaches reveal emergent properties of the global metabolic connectivity. We highlight how the integration of both approaches promises to deliver insights on the structure and function of metabolic systems with wide-ranging implications in discovery science, precision medicine and industrial biotechnology.
A fundamental question in biology is how cell populations evolve into different subtypes based on homogeneous processes at the single cell level. Here we show that population bimodality can emerge even when biological processes are homogenous at the cell level and the environment is kept constant. Our model is based on the stochastic partitioning of a cell component with an optimal copy number. We show that the existence of unimodal or bimodal distributions depends on the variance of partition errors and the growth rate tolerance around the optimal copy number. In particular, our theory provides a consistent explanation for the maintenance of aneuploid states in a population. The proposed model can also be relevant for other cell components such as mitochondria and plasmids, whose abundances affect the growth rate and are subject to stochastic partition at cell division.
157 - Jing Zhao , Guo-Hui Ding , Lin Tao 2007
The architecture of biological networks has been reported to exhibit high level of modularity, and to some extent, topological modules of networks overlap with known functional modules. However, how the modular topology of the molecular network affec ts the evolution of its member proteins remains unclear. In this work, the functional and evolutionary modularity of Homo sapiens (H. sapiens) metabolic network were investigated from a topological point of view. Network decomposition shows that the metabolic network is organized in a highly modular core-periphery way, in which the core modules are tightly linked together and perform basic metabolism functions, whereas the periphery modules only interact with few modules and accomplish relatively independent and specialized functions. Moreover, over half of the modules exhibit co-evolutionary feature and belong to specific evolutionary ages. Peripheral modules tend to evolve more cohesively and faster than core modules do. The correlation between functional, evolutionary and topological modularity suggests that the evolutionary history and functional requirements of metabolic systems have been imprinted in the architecture of metabolic networks. Such systems level analysis could demonstrate how the evolution of genes may be placed in a genome-scale network context, giving a novel perspective on molecular evolution.
Despite their topological complexity almost all functional properties of metabolic networks can be derived from steady-state dynamics. Indeed, many theoretical investigations (like flux-balance analysis) rely on extracting function from steady states . This leads to the interesting question, how metabolic networks avoid complex dynamics and maintain a steady-state behavior. Here, we expose metabolic network topologies to binary dynamics generated by simple local rules. We find that the networks response is highly specific: Complex dynamics are systematically reduced on metabolic networks compared to randomized networks with identical degree sequences. Already small topological modifications substantially enhance the capacity of a network to host complex dynamic behavior and thus reduce its regularizing potential. This exceptionally pronounced regularization of dynamics encoded in the topology may explain, why steady-state behavior is ubiquitous in metabolism.
التعليقات
جاري جلب التعليقات جاري جلب التعليقات
سجل دخول لتتمكن من متابعة معايير البحث التي قمت باختيارها
mircosoft-partner

هل ترغب بارسال اشعارات عن اخر التحديثات في شمرا-اكاديميا