The purpose of this research was to determine the effect of the corpus luteum in
various stages on morphological characteristics of cumulus oocyte complex consequently
on the yield of morphologically intact oocytes for in vitro culturing.
Oocytes
were collected from cow ovariesof animals after slaughter.
Ovaries were divided into the following four groups: (a) ovaries with corpus
hemorrhagicum; (b) ovaries with a functional corpus luteum;
(c) ovaries with regressing corpus luteum (albicans) ;( d) ovaries without any luteal
structure.
The results of this research show that, the intact oocytes yield for in vitro culturing
differs accordingly with the stage of the corpus luteum.
The highest percentage of intact cell(8.1 ± 1.6) were recorded in ovaries without
corpus luteum , whereas the lowest percentage of intact cells were recorded in ovaries with
regressing corpus luteum(5.5 ± 1.3). There was significant difference (p < 0.001)between
the two groups for intact cells.
The aim of this research is to determine the effect of seasonal changes on the
yield of morphologically intact oocytes for in vitro maturation and consequently
for fertilization and embryo production. Determining the impact of seasonal
changes hel
ps us to increase the intact oocytes yield, and better use of in vitro
culturing technique of cow oocytes. This leads to: benefit from animals of
genetic superiority, maintenance the biodiversity of animal genetic resources by
using deep-freezing technique of pre/post in vitro fertilized oocytes taken from
cow ovary of animals after slaughter.
The results of this research paper show that, the intact oocytes yield for in
vitro culturing differs accordingly with year seasons. The lowest percentage of
intact cells 29.5 were recorded in the period between May and September,
whereas the highest percentage of intact cells 42.6% were recorded in the
period between October and March. There was significant difference (P<0.001)
between the two periods for intact cells.
