Vitamin C concentration was studied in samples of infant formula from 5 companies
(referenced A-E) using the 2,6-Dichlorophenolindophenol titration (AOAC, 2006) method.
Results revealed that ascorbic acid content in formulas D and C was the closest
to its
recommended daily intake (RDI) and to the concentrations which are mentioned on the
label of the samples. Storage conditions at room temperature or in the refrigerator did not
affect ascorbic acid levels. In comparison to vitamin C concentrations in milk dissolved in
tap water, its levels decreased when water was warm (40°C) or hot (100°C), and its
concentrations further declined in dissolved form kept for two hours after preparation.