The prevalence of gastric intestinal metaplasia through upper gastrointestinal endoscopy and histological study, and association with Helicobacter pylori in the university hospitals of Tishreen university

gastric intestinal metaplasia (GIM) is considered to be a precursor of gastric cancer which is still the fourth most common cancer and second leading cause of cancer related death worldwide after lung cancer. There is also a relationship between gastric intestinal metaplasia and H.pylory which is one of the most important factors predisposing it . As a result, H. pylori eradication is one of the most promising approaches in gastric cancer prevention. The aim of the study was investigated the prevalence of GIM and its subtypes, and the prevalence of H. pylori infection in GIM. the study was conducted on 200 patients who were undergoing esophagogastroduodenoscopy (EGD) with biopsy between January 2016 and April 2017, in the university hospitals of Tishreen university. Specimens were stained with hematoxylin and eosin to determine the pattern of intestinal metaplasia. And Helicobacter pylori was investigated by Giemsa staining. The prevalence of GIM was 16%. The prevalence of complete IM was higher than incomplete IM. The prevalence of H. pylori infection was 50% in GIM, without presence of statistical importance ) P > 0.05). The prevalence of chronic gastritis was 100% in GIM (P Value = 0.02). Gastric intestinal metaplasia was more prevalent in older patients. GIM is a common finding in patients undergoing EGD with biopsy in our region. Chronic gastritis is considered as the most important reason for the development of GIM.

Screening of Serum Anti-Helicobacter pylori Antibodies in Patients with Peptic Complaints Using Indirect Immunofluorescence Assay

The study aims to determine the Seroprevalence of anti-H. pylori antibodies in patients with peptic complaints using indirect immunofluorescence assay (IFA), and compare serology with histological examination of biopsies from upper gastrointestinal endoscopy to evaluate the sensitivity and specificity of this assay. The Study sample included 120 participants divided into two groups: 69 patients with peptic complaints who underwent upper GI endoscopy in Al-Assad Hospital in Lattakia (average age 47 years old), and 51 volunteers without peptic complaints (average age 33 years old). The study was conducted in the period between February 2011 and March 2012. Indirect immunofluorescence assay (IFA) was used to study the seroprevalence of anti-H. pylori antibodies and fluorescence intensity in the two groups. The results showed that 63 of the patients group (91.3%) and 39 of volunteers (76.47%) are anti-H. pylori antibodies positive. The comparison of serology with histological examination of biopsies showed a sensitivity and specificity of 78% and 80% respectively in the presence of moderate and strong degree of fluorescence. The results reveal the importance of IFA as a non-invasive screening test besides the endoscopy, and the utility of this technique for the epidemiologic studies in a local setting.

The Role of Helicobacter Pylori in Gastritis and Gastroduodenitis in Children

The aim of this study is to determine the incidence of helicobacter pylori (HP) in patients with chronic gastroduodenitis and to compare the clinical presentations between patients with positive HP chronic gastroduodenitis and those with negative HP. Also, To compare the endoscopic findings between patients with HP positive gastroduodenitis and HP negative children. Gastroduodenoscopy was done for every child with abdominal pain admitted at the children hospital-Damascus University between 2002-2003. Mucosal biopsy was taken from every patient who had gastroduodenitis diagnosed by gastroduodenoscopy. The percentage of patients with gastroduodenitis having HP positive erosions was 48.38 %. Higher percentage of HP positive patient was more present in those with the erosion and sub-atrophy forms.