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Imaging and analysis of many single cells hold great potential in our understanding of heterogeneous and complex life systems and in enabling biomedical applications. We here introduce a recently realized image-free imaging cytometry technology, which we call ghost cytometry. While a compressive ghost imaging technique utilizing objects motion relative to a projected static light pattern allows recovery of their images, a key of this ghost cytometry is to achieve ultrafast cell classification by directly applying machine learning methods to the compressive imaging signals in a temporal domain. We show the applicability of our method in the analysis of flowing objects based on the reconstructed images as well as in that based on the imaging waveform without image production.
Computational ghost imaging is an imaging technique in which an object is imaged from light collected using a single-pixel detector with no spatial resolution. Recently, ghost cytometry has been proposed for a high-speed cell-classification method th
We introduce an image cytometer (I-CYT) for the analysis of phytoplankton in fresh and marine water environments. A linear quantification of cell numbers was observed covering several orders of magnitude using cultures of Tetraselmis and Nannochlorop
In this paper we consider a statistical estimation problem known as atomic deconvolution. Introduced in reliability, this model has a direct application when considering biological data produced by flow cytometers. In these experiments, biologists me
Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been increasing interes
One of the key limitations in conventional deep learning based image reconstruction is the need for registered pairs of training images containing a set of high-quality groundtruth images. This paper addresses this limitation by proposing a novel uns