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Theoretical Modeling Expressions for Networked Enzymatic Signal Processing Steps as Logic Gates Optimized by Filtering

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 نشر من قبل Vladimir Privman
 تاريخ النشر 2016
  مجال البحث علم الأحياء فيزياء
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 تأليف Vladimir Privman




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We describe modeling approaches to a network of connected enzyme-catalyzed reactions, with added (bio)chemical processes that introduce biochemical filtering steps into the functioning of such a biocatalytic cascade. Theoretical expressions are derived that allow simple, few-parameter modeling of processes concatenated in such cascades, both with and without filtering. The modeling approach captures and explains features identified in earlier studies of enzymatic processes considered as potential network components for multi-step information/signal processing systems.



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We report the first study of a network of connected enzyme-catalyzed reactions, with added chemical and enzymatic processes that incorporate the recently developed biochemical filtering steps into the functioning of this biocatalytic cascade. New the oretical expressions are derived to allow simple, few-parameter modeling of network components concatenated in such cascades, both with and without filtering. The derived expressions are tested against experimental data obtained for the realized networks responses, measured optically, to variations of its input chemicals concentrations with and without filtering processes. We also describe how the present modeling approach captures and explains several observations and features identified in earlier studies of enzymatic processes when they were considered as potential network components for multi-step information/signal processing systems.
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