اشترك بالحزمة الذهبية واحصل على وصول غير محدود شمرا أكاديميا
تسجيل مستخدم جديدNon-invasive super-resolution imaging through scattering media using fluctuating speckles
343
0
0.0
(
0
)
اسأل ChatGPT حول البحث
ﻻ يوجد ملخص باللغة العربية
Extending super-resolution imaging techniques to objects hidden in strongly scattering media potentially revolutionize the technical analysis for much broader categories of samples, such as biological tissues. The main challenge is the medias inhomogeneous structures which scramble the light path and create noise-like speckle patterns, hindering the objects visualization even at a low-resolution level. Here, we propose a computational method relying on the objects spatial and temporal fluctuation to visualize nanoscale objects through scattering media non-invasively. The fluctuating object can be achieved by random speckle illumination, illuminating through dynamic scattering media, or flickering emitters. The optical memory effect allows us to derive the object at diffraction limit resolution and estimate the point spreading function (PSF). Multiple images of the fluctuating object are obtained by deconvolution, then super-resolution images are achieved by computing the high order cumulants. Non-linearity of high order cumulant significantly suppresses the noise and artifacts in the resulting images and enhances the resolution by a factor of $sqrt{N}$, where $N$ is the cumulant order. Our non-invasive super-resolution speckle fluctuation imaging (NISFFI) presents a nanoscopy technique with very simple hardware to visualize samples behind scattering media.
قيم البحث
اقرأ أيضاً
Super-resolution imaging with advanced optical systems has been revolutionizing technical analysis in various fields from biological to physical sciences. However, many objects are hidden by strongly scattering media such as rough wall corners or bio
logical tissues that scramble light paths, create speckle patterns and hinder objects visualization, let alone super-resolution imaging. Here, we realize a method to do non-invasive super-resolution imaging through scattering media based on stochastic optical scattering localization imaging (SOSLI) technique. Simply by capturing multiple speckle patterns of photo-switchable emitters in our demonstration, the stochastic approach utilizes the speckle correlation properties of scattering media to retrieve an image with more than five-fold resolution enhancement compared to the diffraction limit, while posing no fundamental limit in achieving higher spatial resolution. More importantly, we demonstrate our SOSLI to do non-invasive super-resolution imaging through not only optical diffusers, i.e. static scattering media, but also biological tissues, i.e. dynamic scattering media with decorrelation of up to 80%. Our approach paves the way to non-invasively visualize various samples behind scattering media at unprecedented levels of detail.
On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and inside scatte
ring layers across a large field of view remains elusive due to the physical limits set by the optical memory effect, especially without wavefront shaping techniques. Here, we demonstrate an approach that enables non-invasive fluorescence imaging behind scattering layers with field-of-views extending well beyond the optical memory effect. The method consists in demixing the speckle patterns emitted by a fluorescent object under variable unknown random illumination, using matrix factorization and a novel fingerprint-based reconstruction. Experimental validation shows the efficiency and robustness of the method with various fluorescent samples, covering a field of view up to three times the optical memory effect range. Our non-invasive imaging technique is simple, neither requires a spatial light modulator nor a guide star, and can be generalized to a wide range of incoherent contrast mechanisms and illumination schemes.
We develop a method based on the cross-spectrum of an intensity-modulated CW laser, which can extract a signal from an extremely noisy environment and image objects hidden in turbid media. We theoretically analyzed our scheme and performed the experi
ment by scanning the object placed in between two ground glass diffusers. The image of the object is retrieved by collecting the amplitudes at the modulation frequency of all the cross-spectra. Our method is non-invasive, easy-to-implement, and can work for both static and dynamic media.
Imaging through scattering media is a useful and yet demanding task since it involves solving for an inverse mapping from speckle images to object images. It becomes even more challenging when the scattering medium undergoes dynamic changes. Various
approaches have been proposed in recent years. However, to date, none is able to preserve high image quality without either assuming a finite number of sources for dynamic changes, assuming a thin scattering medium, or requiring the access to both ends of the medium. In this paper, we propose an adaptive inverse mapping (AIP) method which is flexible regarding any dynamic change and only requires output speckle images after initialization. We show that the inverse mapping can be corrected through unsupervised learning if the output speckle images are followed closely. We test the AIP method on two numerical simulations, namely, a dynamic scattering system formulated as an evolving transmission matrix and a telescope with a changing random phase mask at a defocus plane. Then we experimentally apply the AIP method on a dynamic fiber-optic imaging system. Increased robustness in imaging is observed in all three cases. With the excellent performance, we see the great potential of the AIP method in imaging through dynamic scattering media.
This work presents a new super-resolution imaging approach by using subwavelength hole resonances. We employ a subwavelength structure in which an array of tiny holes are etched in a metallic slab with the neighboring distance $ell$ that is smaller t
han half of the wavelength. By tuning the incident wave at resonant frequencies, the subwavelength structure generates strong illumination patterns that are able to probe both low and high spatial frequency components of the imaging sample sitting above the structure. The image of the sample is obtained by performing stable numerical reconstruction from the far-field measurement of the diffracted wave. It is demonstrated that a resolution of $ell/2$ can be obtained for reconstructed images, thus one can achieve super-resolution by arranging multiple holes within one wavelength. The proposed approach may find applications in wave-based imaging such as electromagnetic and ultrasound imaging. It attains two advantages that are important for practical realization. It avoids the difficulty to control the distance the between the probe and the sample surface with high precision. In addition, the numerical reconstructed images are very stable against noise by only using the low frequency band of the far-field data in the numerical reconstruction.
سجل دخول لتتمكن من نشر تعليقات
التعليقات
جاري جلب التعليقات
سجل دخول لتتمكن من متابعة معايير البحث التي قمت باختيارها
