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The OpenFlexure Microscope is a 3D printed, low-cost microscope capable of automated image acquisition through the use of a motorised translation stage and a Raspberry Pi imaging system. This automation has applications in research and healthcare, including in supporting the diagnosis of malaria in low resource settings. The plasmodium parasites which cause malaria require high magnification imaging, which has a shallow depth of field, necessitating the development of an accurate and precise autofocus procedure. We present methods of identifying the focal plane of the microscope, and procedures for reliably acquiring a stack of focused images on a system affected by backlash and drift. We also present and assess a method to verify the success of autofocus during the scan. The speed, reliability and precision of each method is evaluated, and the limitations discussed in terms of the end users requirements.
We introduce a wide field hyperspectral microscope using the Fourier-transform approach. The interferometer is based on the Translating-Wedge-Based Identical Pulses eNcoding System (TWINS) [Opt. Lett. 37, 3027 (2012)], a common-path birefringent inte
We describe the design, construction, and performance of an ultra-high vacuum (UHV) scanning tunneling microscope (STM) capable of imaging at dilution-refrigerator temperatures and equipped with a vector magnet. The primary objective of our design is
Imaging and manipulating individual atoms with submicrometer separation can be instrumental for quantum simulation of condensed matter Hamiltonians and quantum computation with neutral atoms. Quantum gas microscope experiments in most cases rely on q
Conventional microscope objective lenses are diffraction limited, which means that they cannot resolve features smaller than half the illumination wavelength. Under white light illumination, such resolution limit is about 250-300 nm for an ordinary m
Dedicated accelerometers have been developed for the MICROSCOPE mission taking into account the specific range of acceleration to be measured on board the satellite. Considering one micro-g and even less as the full range of the instrument, leads to