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Absorption cells filled with diatomic iodine are frequently employed as wavelength reference for high-precision stellar radial velocity determination due their long-term stability and low cost. Despite their wide-spread usage in the community, there is little documentation on how to determine the ideal operating temperature of an individual cell. We have developed a new approach to measuring the effective molecular temperature inside a gas absorption cell and searching for effects detrimental to a high precision wavelength reference, utilizing the Boltzmann distribution of relative line depths within absorption bands of single vibrational transitions. With a high resolution Fourier transform spectrometer, we took a series of 632 spectra at temperatures between 23{deg}C and 66{deg}C. These spectra provide a sufficient basis to test the algorithm and demonstrate the stability and repeatability of the temperature determination via molecular lines on a single iodine absorption cell. The achievable radial velocity precision is found to be independent of the cell temperature and a detailed analysis shows a wavelength dependency, which originates in the resolving power of the spectrometer in use and the signal-to-noise ratio. Two effects were found to cause apparent absolute shifts in radial velocity, a temperature-induced shift of the order of 1 m/s/K and a more significant effect resulting in abrupt jumps of 50 m/s is determined to be caused by the temperature crossing the dew point of the molecular iodine.
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