ﻻ يوجد ملخص باللغة العربية
Unravelling the physical mechanisms behind the organisation of lipid domains is a central goal in cell biology and membrane biophysics. Previous studies on cells and model lipid bilayers featuring phase-separated domains found an intricate interplay between the membrane geometry and its chemical composition. However, the lack of a model system with simultaneous control over the membrane shape and conservation of its composition precluded a fundamental understanding of curvature-induced effects. Here, we present a new class of multicomponent vesicles supported by colloidal scaffolds of designed shape. We find that the domain composition adapts to the geometry, giving rise to a novel antimixed state. Theoretical modelling allowed us to link the pinning of domains by regions of high curvature to the material parameters of the membrane. Our results provide key insights into the phase separation of cellular membranes and on curved surfaces in general.
Many biological systems fold thin sheets of lipid membrane into complex three-dimensional structures. This microscopic origami is often mediated by the adsorption and self-assembly of proteins on a membrane. As a model system to study adsorption-medi
Experiments on supported lipid bilayers featuring liquid ordered/disordered domains have shown that the spatial arrangement of the lipid domains and their chemical composition are strongly affected by the curvature of the substrate. Furthermore, theo
Soft bodies flowing in a channel often exhibit parachute-like shapes usually attributed to an increase of hydrodynamic constraint (viscous stress and/or confinement). We show that the presence of a fluid membrane leads to the reverse phenomenon and b
We investigated the phase separation of dioleoylphosphatidylserine (DOPS) and dipalmitoylphosphatidylcholine (DPPC) in giant unilamellar vesicles in hypotonic solution using fluorescence and confocal laser scanning microscopy. Although phase separati
Necks are features of lipid membranes characterized by an uniquley large curvature, functioning as bridges between different compartments. These features are ubiquitous in the life-cycle of the cell and instrumental in processes such as division, ext